ArticleDiabetes2012
In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.
Article in Diabetes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
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- The nitrone compound OKN-007 delays motor neuron loss and disease progression in the G93A mouse model of amyotrophic lateral sclerosis.Frontiers in neuroscience · 2024Article
- Oklahoma Nathan Shock Aging Center - assessing the basic biology of aging from genetics to protein and function.GeroScience · 2021Article
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- Assessing bladder hyper-permeability biomarkersAmerican journal of nuclear medicine and molecular imaging · 2020Article
- Using MRI to measure in vivo free radical production and perfusion dynamics in a mouse model of elevated oxidative stress and neurogenic atrophy.Redox biology · 2019Article
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- Streptozotocin‑induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization.Molecular medicine reports · 2018Article
- Oxidative stress measured in vivo without an exogenous contrast agent using QUEST MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Review
- Assessing long-term neuroinflammatory responses to encephalopathy using MRI approaches in a rat endotoxemia model.GeroScience · 2018Article
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association.Circulation research · 2016Review
- Imaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping.Redox biology · 2016Review
- OKN-007 decreases free radical levels in a preclinical F98 rat glioma model.Free radical biology & medicine · 2015Article
- Autophagy Mediates Astrocyte Death During Zinc-Potentiated Ischemia--Reperfusion Injury.Biological trace element research · 2015Article
- Article
- In vivo targeted molecular magnetic resonance imaging of free radicals in diabetic cardiomyopathy within mice.Free radical research · 2015Article
- Immuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals.Biochimica et biophysica acta · 2014Review
- Imaging Neuroinflammation - from Bench to Bedside.Journal of clinical & cellular immunology · 2014Article
- Nitrone-based therapeutics for neurodegenerative diseases: their use alone or in combination with lanthionines.Free radical biology & medicine · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Oxidative stress plays a major role in diabetes. In vivo levels of membrane-bound radicals (MBRs) in a streptozotocin-induced diabetic mouse model were uniquely detected by combining molecular magnetic resonance imaging (mMRI) and immunotrapping techniques. An anti-DMPO (5,5-dimethyl-1-pyrroline N-oxide) antibody (Ab) covalently bound to an albumin (BSA)-Gd (gadolinium)-DTPA (diethylene triamine penta acetic acid)-biotin MRI contrast agent (anti-DMPO probe), and mMRI, were used to detect in vivo levels of DMPO-MBR adducts in kidneys, livers, and lungs of diabetic mice, after DMPO administration. Magnetic resonance signal intensities, which increase in the presence of a Gd-based molecular probe, were significantly higher within the livers, kidneys, and lungs of diabetic animals administered the anti-DMPO probe compared with controls. Fluorescence images validated the location of the anti-DMPO probe in excised tissues via conjugation of streptavidin-Cy3, which targeted the probe biotin moiety, and immunohistochemistry was used to validate the presence of DMPO adducts in diabetic mouse livers. This is the first report of noninvasively imaging in vivo levels of MBRs within any disease model. This method can be specifically applied toward diabetes models for in vivo assessment of free radical levels, providing an avenue to more fully understand the role of free radicals in diabetes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.