ArticleJournal of proteome research2012
Human cornea proteome: identification and quantitation of the proteins of the three main layers including epithelium, stroma, and endothelium.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 1 citations in OpenAlex.
- A multi-ethnic genome-wide association study implicates collagen matrix integrity and cell differentiation pathways in keratoconus.Communications biology · 2021Pooled it
- Reframing Technology and Ethics in Corneal Xenotransplantation: A Scoping Review of Standards and Safety in Ophthalmology.Health care science · 2026Review
- Cryo-EM structure of TGFBIp fibrils driven by a corneal dystrophy-linked mutation enables design of peptide inhibitors of aggregation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Latanoprost Acid-Brimonidine, a New Amide Prodrug for Glaucoma Management Based on the Concept of Sustained Release.Molecules (Basel, Switzerland) · 2026Article
- Layer-Specific Proteomic Profiling of the Human Cornea Reveals Insights Into Structure and Biological Function.Investigative ophthalmology & visual science · 2026Article
- A Comprehensive Proteome of Human Corneal Epithelial Cells Constructed by Cross-platform DIA-Mass Spectrometry.Scientific data · 2025Article
- Biochemical components of corneal stroma: a study on myopia classification based on Raman spectroscopy and deep learning methods.Biomedical optics express · 2025Article
- Transcriptomic landscape of quiescent and proliferating human corneal stromal fibroblasts.Experimental eye research · 2024Article
- Biochemical component analysis of human myopic corneal stroma using the Raman spectrum.International ophthalmology · 2024Article
- Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Article
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
- Recent Approaches to the Modification of Collagen Biomatrix as a Corneal Biomatrix and Its Cellular Interaction.Polymers · 2023Review
- Release of frustration drives corneal amyloid disaggregation by brain chaperone.Communications biology · 2023Article
- Autophagy in the normal and diseased cornea.Experimental eye research · 2022Review
- Ocular proteomic and transcriptomic changes with aging in a rabbit model of lensectomy with intraocular lens insertion.Experimental eye research · 2022Article
- Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior.Biomedicines · 2022Article
- Shotgun Proteomics for the Identification and Profiling of the Tear Proteome of Keratoconus Patients.Investigative ophthalmology & visual science · 2022Article
- Proteome Analysis of Whole-Body Responses in Medaka Experimentally Exposed to Fish-Killing DinoflagellateInternational journal of molecular sciences · 2021Article
- Molecular characteristics and spatial distribution of adult human corneal cell subtypes.Scientific reports · 2021Article
- Human Cadaveric Donor Cornea Derived Extra Cellular Matrix Microparticles for Minimally Invasive Healing/Regeneration of Corneal Wounds.Biomolecules · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Diseases of the cornea are common and refer to conditions like infections, injuries and genetic defects. Morphologically, many corneal diseases affect only certain layers of the cornea and separate analysis of the individual layers is therefore of interest to explore the basic molecular mechanisms involved in corneal health and disease. In this study, the three main layers including, the epithelium, stroma and endothelium of healthy human corneas were isolated. Prior to analysis by LC-MS/MS the proteins from the different layers were either (i) separated by SDS-PAGE followed by in-gel trypsinization, (ii) in-solution digested without prior protein separation or, (iii) in-solution digested followed by cation exchange chromatography. A total of 3250 unique Swiss-Prot annotated proteins were identified in human corneas, 2737 in the epithelium, 1679 in the stroma, and 880 in the endothelial layer. Of these, 1787 proteins have not previously been identified in the human cornea by mass spectrometry. In total, 771 proteins were quantified, 157 based on in-solution digestion and 770 based on SDS-PAGE separation followed by in-gel digestion of excised gel pieces. Protein analysis showed that many of the identified proteins are plasma proteins involved in defense responses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.