ArticleAnalytical chemistry2012
Ultrasensitive detection of cancer biomarkers in the clinic by use of a nanostructured microfluidic array.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it, 203 citations in OpenAlex.
- Diagnostic Efficacy of Serum Biomarkers for Oral Cancer: An Updated Systematic Review and Meta-Analysis.Asian Pacific journal of cancer prevention : APJCP · 2026Pooled it
- Redefining oropharyngeal cancer in the HPV era: integrating precision medicine and immunotherapeutic frontiers.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026Review
- Emerging roles of microfluidics in oral cancer research and clinical translation.Materials today. Bio · 2026Review
- Research progress on carcinogenic factors and personalized treatment of oral cancer.Discover oncology · 2025Review
- Review
- Visualizing cancer resistance via nano-quenching and recovery detector of CD44.Journal of nanobiotechnology · 2024Article
- Sandwich-like voltametric immunosensing of interleukin-8 based on β-cyclodextrin/carbon nanotubes and methylthionine chloride@UIO-66 framework.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Mapping the Potential of Microfluidics in Early Diagnosis and Personalized Treatment of Head and Neck Cancers.Cancers · 2023Review
- Review
- MoSDiagnostics (Basel, Switzerland) · 2023Article
- Advanced materials and technologies for oral diseases.Science and technology of advanced materials · 2023Review
- A Microfluidic Platform Revealing Interactions between Leukocytes and Cancer Cells on Topographic Micropatterns.Biosensors · 2022Article
- Review
- Molecular Biomarkers in Cancer.Biomolecules · 2022Review
- Microfluidic Systems for Cancer Diagnosis and Applications.Micromachines · 2021Review
- Biosensors Designed for Clinical Applications.Biomedicines · 2021Review
- Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments.Cancers · 2021Review
- Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices.Sensors (Basel, Switzerland) · 2021Review
- Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray.Biosensors & bioelectronics · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 8 institutions in 3 countries.
Funding
Abstract
Multiplexed biomarker protein detection holds unrealized promise for clinical cancer diagnostics due to lack of suitable measurement devices and lack of rigorously validated protein panels. Here we report an ultrasensitive electrochemical microfluidic array optimized to measure a four-protein panel of biomarker proteins, and we validate the protein panel for accurate oral cancer diagnostics. Unprecedented ultralow detection into the 5-50 fg·mL(-1) range was achieved for simultaneous measurement of proteins interleukin 6 (IL-6), IL-8, vascular endothelial growth factor (VEGF), and VEGF-C in diluted serum. The immunoarray achieves high sensitivity in 50 min assays by using off-line protein capture by magnetic beads carrying 400,000 enzyme labels and ~100,000 antibodies. After capture of the proteins and washing to inhibit nonspecific binding, the beads are magnetically separated and injected into the array for selective capture by antibodies on eight nanostructured sensors. Good correlations with enzyme-linked immunosorbent assays (ELISA) for protein determinations in conditioned cancer cell media confirmed the accuracy of this approach. Normalized means of the four protein levels in 78 oral cancer patient serum samples and 49 controls gave clinical sensitivity of 89% and specificity of 98% for oral cancer detection, demonstrating high diagnostic utility. The low-cost, easily fabricated immunoarray provides a rapid serum test for diagnosis and personalized therapy of oral cancer. The device is readily adaptable to clinical diagnostics of other cancers.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.