ArticleStem cells (Dayton, Ohio)2012
A comparative transcriptomic analysis reveals conserved features of stem cell pluripotency in planarians and mammals.
Article in Stem cells (Dayton, Ohio), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
114 citing papers in PubMed, 208 citations in OpenAlex.
- A Tousled-like kinase (TLK) homolog required for male germline stem cell homeostasis in the planarian Schmidtea mediterranea.Developmental biology · 2026Article
- Cotyledon opening during seedling deetiolation is determined by ABA-mediated splicing regulation.EMBO reports · 2025Article
- A PIF-regulated switch in cell axis growth drives cotyledon expansion through tissue-specific cell expansion and division.The Plant journal : for cell and molecular biology · 2025Article
- Drosophila ovarian stem cell niche ageing involves coordinated changes in transcription and alternative splicing.Nature communications · 2025Article
- Chromatin remodeling protein BPTF mediates chromatin accessibility at gene promoters in planarian stem cells.BMC genomics · 2025Article
- Regeneration in the absence of canonical neoblasts in an early branching flatworm.Nature communications · 2025Article
- Stem cells (neoblasts) and positional information jointly dominate regeneration in planarians.Heliyon · 2025Review
- Mining of potentially stem cell-related miRNAs in planarians.Molecular biology reports · 2024Review
- Regeneration in the absence of canonical neoblasts in an early branching flatworm.bioRxiv : the preprint server for biology · 2024Article
- Annelid adult cell type diversity and their pluripotent cellular origins.Nature communications · 2024Article
- Purification of Planarian Stem Cells Using a Draq5-Based FACS Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Purification and transcriptomic characterization of proliferative cells ofFrontiers in parasitology · 2024Article
- LIM-HD transcription factors control axial patterning and specify distinct neuronal and intestinal cell identities in planarians.Open biology · 2023Article
- The BAF chromatin remodeling complex licenses planarian stem cells access to ectodermal and mesodermal cell fates.BMC biology · 2023Article
- aristaless-like homeobox-3 is wound induced and promotes a low-Wnt environment required for planarian head regeneration.Development (Cambridge, England) · 2023Article
- Alternative Splicing Changes Promoted by NOVA2 Upregulation in Endothelial Cells and Relevance for Gastric Cancer.International journal of molecular sciences · 2023Article
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- Pluripotency retention and exogenous mRNA introduction in planarian stem cells in culture.iScience · 2023Article
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- Regulation of alternative splicing by retrograde and light signals converges to control chloroplast proteins.Frontiers in plant science · 2023Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Many long-lived species of animals require the function of adult stem cells throughout their lives. However, the transcriptomes of stem cells in invertebrates and vertebrates have not been compared, and consequently, ancestral regulatory circuits that control stem cell populations remain poorly defined. In this study, we have used data from high-throughput RNA sequencing to compare the transcriptomes of pluripotent adult stem cells from planarians with the transcriptomes of human and mouse pluripotent embryonic stem cells. From a stringently defined set of 4,432 orthologs shared between planarians, mice and humans, we identified 123 conserved genes that are ≥5-fold differentially expressed in stem cells from all three species. Guided by this gene set, we used RNAi screening in adult planarians to discover novel stem cell regulators, which we found to affect the stem cell-associated functions of tissue homeostasis, regeneration, and stem cell maintenance. Examples of genes that disrupted these processes included the orthologs of TBL3, PSD12, TTC27, and RACK1. From these analyses, we concluded that by comparing stem cell transcriptomes from diverse species, it is possible to uncover conserved factors that function in stem cell biology. These results provide insights into which genes comprised the ancestral circuitry underlying the control of stem cell self-renewal and pluripotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.