ArticleMolecular biology reports2012
Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Cloning of the Arabidopsis SMAP2 promoter and analysis of its expression activity.Scientific reports · 2024Article
- Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean.Transgenic research · 2021Article
- Molecular basis of natural tolerance to glyphosate in Convolvulus arvensis.Scientific reports · 2019Article
- Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (International journal of molecular sciences · 2018Article
- Analysis of tandem repeat units of the promoter of capsanthin/capsorubin synthase (Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.BMC plant biology · 2014Article
- Battle through signaling between wheat and the fungal pathogen Septoria tritici revealed by proteomics and phosphoproteomics.Molecular & cellular proteomics : MCP · 2013Article
- Design of optimal solvent for extraction of bio-active ingredients from six varieties of Medicago sativa.Chemistry Central journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 1272 bp upstream sequence of MsZFN gene was cloned from alfalfa, which was designed as MsZPP (Genbank accession number: FJ 161979.2) using an adaptor-mediated genome walking method. A sole transcription start site was located 69 bp upstream of the translation start site. Its pattern of expression included roots, stem vascular tissues, floral reproductive organs, and leaves, but the promoter did not express in seeds, petals or sepals. Transcription levels can be stimulated by dark, MeJA, and IAA. However, GUS fusion activities had no change by treatments of GA, ABA, drought and high salt for 3 days. Deletion analysis revealed that all sections of the promoter can drive gus gene expression in the root, stem, leaves and floral reproductive organs; however, only fragments longer than the -460 bp promoter can stimulate strong gus gene expression in these organs. In addition, the -460 bp promoter fragment can drive gus expression not only in the vascular tissue, but also in leaf guard cells. The results suggest that the promoter MsZPP plays roles in the regulation of transgene expression, particularly due to its darkness, MeJA, and IAA responsiveness.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.