ArticleACS nano2012
FRET-labeled siRNA probes for tracking assembly and disassembly of siRNA nanocomplexes.
Article in ACS nano, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Fluorescent Labeling of Inulin-Based siRNA Delivery Nanocarriers: Implications for Stability and Biological Performance.ACS omega · 2026Article
- Evaluation of siRNA Stability and Interaction with Serum Components Using an Agarose Gel-Based Single-Molecule FRET Labeling Method.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Development of FRET-based high-throughput screening for viral RNase III inhibitors.Molecular plant pathology · 2020Article
- Simple FRET Electrophoresis Method for Precise and Dynamic Evaluation of Serum siRNA Stability.ACS medicinal chemistry letters · 2020Article
- Delivery Pathway Regulation of 3',3″-Bis-Peptide-siRNA Conjugate via Nanocarrier Architecture Engineering.Molecular therapy. Nucleic acids · 2018Article
- Nanoparticles for siRNA-Based Gene Silencing in Tumor Therapy.IEEE transactions on nanobioscience · 2016Review
- Functional polyesters enable selective siRNA delivery to lung cancer over matched normal cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Intracellular trafficking and exocytosis of a multi-component siRNA nanocomplex.Nanomedicine : nanotechnology, biology, and medicine · 2016Article
- Hydrolytic charge-reversal of PEGylated polyplexes enhances intracellular un-packaging and activity of siRNA.Journal of biomedical materials research. Part A · 2016Article
- Live cell imaging of duplex siRNA intracellular trafficking.Nucleic acids research · 2015Article
- Dual secured nano-melittin for the safe and effective eradication of cancer cells.Journal of materials chemistry. B · 2015Article
- Tuning PEGylation of mixed micelles to overcome intracellular and systemic siRNA delivery barriers.Biomaterials · 2015Article
- siRNA nanoparticles: the future of RNAi therapeutics for oncology?Nanomedicine (London, England) · 2014Article
- Insight into mechanisms of cellular uptake of lipid nanoparticles and intracellular release of small RNAs.Pharmaceutical research · 2014Article
- Nanoparticles and their applications in cell and molecular biology.Integrative biology : quantitative biosciences from nano to macro · 2014Review
- Chiral cationic polyamines for chiral microcapsules and siRNA delivery.Bioorganic & medicinal chemistry letters · 2013Article
- Article
- Multiparametric approach for the evaluation of lipid nanoparticles for siRNA delivery.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling.Nature biotechnology · 2013Article
- Investigation of Polyethylenimine/DNA Polyplex Transfection to Cultured Cells Using Radiolabeling and Subcellular Fractionation Methods.Molecular pharmaceutics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The assembly, stability, and timely disassembly of short interfering RNA (siRNA) nanocomplexes have the potential to affect the efficiency of siRNA delivery and gene silencing. As such, the design of new probes that can measure these properties without significantly perturbing the nanocomplexes or their environment may facilitate the study and further development of new siRNA nanocomplexes. Herein, we study Förster resonance energy transfer (FRET)-labeled siRNA probes that can track the assembly, stability, and disassembly of siRNA nanocomplexes in different environments. The probe is composed of two identical siRNAs, each labeled with a fluorophore. Upon nanocomplex formation, the siRNA-bound fluorophores become locally aggregated within the nanocomplex and undergo FRET. A key advantage of this technique is that the delivery vehicle (DV) need not be labeled, thus enabling the characterization of a large variety of nanocarriers, some of which may be difficult or even impossible to label. We demonstrate proof-of-concept by measuring the assembly of various DVs with siRNAs and show good agreement with gel electrophoresis experiments. As a consequence of not having to label the DV, we are able to determine nanocomplex biophysical parameters such as the extracellular apparent dissociation constants (K(D)) and intracellular disassembly half-life for several in-house and proprietary commercial DVs. Furthermore, the lack of DV modification allows for a true direct comparison between DVs as well as correlation between their biophysical properties and gene silencing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.