ArticlePloS one2012
Assembling neurospheres: dynamics of neural progenitor/stem cell aggregation probed using an optical trap.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- A clinically defined and xeno-free hydrogel system for regenerative medicine.Materials futures · 2026Article
- Proneurogenic actions of follicle-stimulating hormone on neurospheres derived from ovarian cortical cells in vitro.BMC veterinary research · 2024Article
- Organization of self-advantageous niche by neural stem/progenitor cells during development via autocrine VEGF-A under hypoxia.Inflammation and regeneration · 2023Article
- Antidepressant Low Doses of Ketamine and Melatonin in Combination Produce Additive Neurogenesis in Human Olfactory Neuronal Precursors.Molecules (Basel, Switzerland) · 2022Article
- Glioblastoma, from disease understanding towards optimal cell-based in vitro models.Cellular oncology (Dordrecht, Netherlands) · 2022Review
- Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries.Science advances · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Evolution of Experimental Models in the Study of Glioblastoma: Toward Finding Efficient Treatments.Frontiers in oncology · 2020Review
- Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers.Scientific reports · 2019Article
- Cell-Cell Connection Enhances Proliferation and Neuronal Differentiation of Rat Embryonic Neural Stem/Progenitor Cells.Frontiers in cellular neuroscience · 2017Article
- Optically trapping tumor cells to assess differentiation and prognosis of cancers.Biomedical optics express · 2016Article
- Characterization of Seeding Conditions for Studies on Differentiation Patterns of Subventricular Zone Derived Neurospheres.Frontiers in cellular neuroscience · 2016Article
- Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy.Journal of biomedical optics · 2015Article
- Preconditioning stem cells for in vivo delivery.BioResearch open access · 2014Review
- Spiral ganglion stem cells can be propagated and differentiated into neurons and glia.BioResearch open access · 2014Article
- Article
- Nardosinone improves the proliferation, migration and selective differentiation of mouse embryonic neural stem cells.PloS one · 2014Article
- Enhancing the strength of an optical trap by truncation.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical trapping (tweezing) has been used in conjunction with fluid flow technology to dissect the mechanics and spatio-temporal dynamics of how neural progenitor/stem cells (NSCs) adhere and aggregate. Hitherto unavailable information has been obtained on the most probable minimum time (∼5 s) and most probable minimum distance of approach (4-6 µm) required for irreversible adhesion of proximate cells to occur. Our experiments also allow us to study and quantify the spatial characteristics of filopodial- and membrane-mediated adhesion, and to probe the functional dynamics of NSCs to quantify a lower limit of the adhesive force by which NSCs aggregate (∼18 pN). Our findings, which we also validate by computational modeling, have important implications for the neurosphere assay: once aggregated, neurospheres cannot disassemble merely by being subjected to shaking or by thermal effects. Our findings provide quantitative affirmation to the notion that the neurosphere assay may not be a valid measure of clonality and "stemness". Post-adhesion dynamics were also studied and oscillatory motion in filopodia-mediated adhesion was observed. Furthermore, we have also explored the effect of the removal of calcium ions: both filopodia-mediated as well as membrane-membrane adhesion were inhibited. On the other hand, F-actin disrupted the dynamics of such adhesion events such that filopodia-mediated adhesion was inhibited but not membrane-membrane adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.