ArticlePloS one2012
Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Biofilm Formation on Endotracheal and Tracheostomy Tubing: A Systematic Review and Meta-Analysis of Culture Data and Sampling Method.MicrobiologyOpen · 2025Pooled it
- Prevention of ventilator-associated pneumonia by metal-coated endotracheal tubes: a meta-analysis.Critical care (London, England) · 2024Pooled it
- Nanostructured Hydrogels: A Method to Prevent Biofilms on Implantable Medical Devices.Gels (Basel, Switzerland) · 2026Review
- Biofilm communities above and below the cuff of endotracheal tubes are spatially homogenous.Respiratory research · 2026Observational
- Fluoroquinolones and Biofilm: A Narrative Review.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Potentially pathogenic culturable bacteria in hemodialysis waters.BMC microbiology · 2024Article
- Description of the colonizing mycobiota of endotracheal tubes from patients admitted to two intensive care units in Bogotá, ColombiaBiomedica : revista del Instituto Nacional de Salud · 2023Article
- Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms ofAntibiotics (Basel, Switzerland) · 2023Article
- Endotracheal tube microbiome in hospitalized patients defined largely by hospital environment.Respiratory research · 2022Article
- Article
- Dentistry and Intensive Care Unit: A Brief Report.European journal of dentistry · 2022Article
- Impact of Healthcare-Associated Infections Connected to Medical Devices-An Update.Microorganisms · 2021Review
- NO donors and NO delivery methods for controlling biofilms in chronic lung infections.Applied microbiology and biotechnology · 2021Review
- Bacteriophage Cocktail-Mediated Inhibition ofAntibiotics (Basel, Switzerland) · 2021Article
- Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention ofMicroorganisms · 2020Article
- Selective digestive decontamination solution used as "lock therapy" prevents and eradicates bacterial biofilm in an in vitro bench-top model.Annals of clinical microbiology and antimicrobials · 2020Article
- Biofilm formation on three different endotracheal tubes: a prospective clinical trial.Critical care (London, England) · 2020Observational
- Quantitative assessment of individual populations within polymicrobial biofilms.Scientific reports · 2018Article
- Endotracheal Tube Biofilm and its Impact on the Pathogenesis of Ventilator-Associated Pneumonia.Journal of critical care medicine (Universitatea de Medicina si Farmacie din Targu-Mures) · 2018Article
- Molecular analysis of endotracheal tube biofilms and tracheal aspirates in the pediatric intensive care unit.Advances in pediatric research · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ventilator-associated pneumonia (VAP) is a common nosocomial infection in mechanically ventilated patients. Biofilm formation is one of the mechanisms through which the endotracheal tube (ET) facilitates bacterial contamination of the lower airways. In the present study, we analyzed the composition of the ET biofilm flora by means of culture dependent and culture independent (16 S rRNA gene clone libraries and pyrosequencing) approaches. Overall, the microbial diversity was high and members of different phylogenetic lineages were detected (Actinobacteria, beta-Proteobacteria, Candida spp., Clostridia, epsilon-Proteobacteria, Firmicutes, Fusobacteria and gamma-Proteobacteria). Culture dependent analysis, based on the use of selective growth media and conventional microbiological tests, resulted in the identification of typical aerobic nosocomial pathogens which are known to play a role in the development of VAP, e.g. Staphylococcus aureus and Pseudomonas aeruginosa. Other opportunistic pathogens were also identified, including Staphylococcus epidermidis and Kocuria varians. In general, there was little correlation between the results obtained by sequencing 16 S rRNA gene clone libraries and by cultivation. Pyrosequencing of PCR amplified 16 S rRNA genes of four selected samples resulted in the identification of a much wider variety of bacteria. The results from the pyrosequencing analysis suggest that these four samples were dominated by members of the normal oral flora such as Prevotella spp., Peptostreptococcus spp. and lactic acid bacteria. A combination of methods is recommended to obtain a complete picture of the microbial diversity of the ET biofilm.
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