ArticlePLoS genetics2012
Histone H3 localizes to the centromeric DNA in budding yeast.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- The ATAD2/ANCCA homolog Yta7 cooperates with Scm3Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Precise genome-wide mapping of single nucleosomes and linkers in vivo.Genome biology · 2018Article
- Novel Centromeric Loci of the Wine and Beer Yeast Dekkera bruxellensis CEN1 and CEN2.PloS one · 2016Article
- Chemical and biological tools for the preparation of modified histone proteins.Topics in current chemistry · 2015Article
- Scm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate.Nucleic acids research · 2014Article
- The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo.eLife · 2014Article
- Kinetochore function and chromosome segregation rely on critical residues in histones H3 and H4 in budding yeast.Genetics · 2013Article
- Review
- Solo or doppio: how many CENP-As make a centromeric nucleosome?Nature structural & molecular biology · 2013Article
- Reconstitution of hemisomes on budding yeast centromeric DNA.Nucleic acids research · 2013Article
- Nucleosomal composition at the centromere: a numbers game.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- The split personality of CENP-A nucleosomes.Cell · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During cell division, segregation of sister chromatids to daughter cells is achieved by the poleward pulling force of microtubules, which attach to the chromatids by means of a multiprotein complex, the kinetochore. Kinetochores assemble at the centromeric DNA organized by specialized centromeric nucleosomes. In contrast to other eukaryotes, which typically have large repetitive centromeric regions, budding yeast CEN DNA is defined by a 125 bp sequence and assembles a single centromeric nucleosome. In budding yeast, as well as in other eukaryotes, the Cse4 histone variant (known in vertebrates as CENP-A) is believed to substitute for histone H3 at the centromeric nucleosome. However, the exact composition of the CEN nucleosome remains a subject of debate. We report the use of a novel ChIP approach to reveal the composition of the centromeric nucleosome and its localization on CEN DNA in budding yeast. Surprisingly, we observed a strong interaction of H3, as well as Cse4, H4, H2A, and H2B, but not histone chaperone Scm3 (HJURP in human) with the centromeric DNA. H3 localizes to centromeric DNA at all stages of the cell cycle. Using a sequential ChIP approach, we could demonstrate the co-occupancy of H3 and Cse4 at the CEN DNA. Our results favor a H3-Cse4 heterotypic octamer at the budding yeast centromere. Whether or not our model is correct, any future model will have to account for the stable association of histone H3 with the centromeric DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.