ArticleJournal of clinical microbiology2012
From Quellung to multiplex PCR, and back when needed, in pneumococcal serotyping.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Molecular Characterization ofMicroorganisms · 2026Article
- Impact and effectiveness of a conjugate vaccine against invasive pneumococcal disease in Finland - a modelling approach.Human vaccines & immunotherapeutics · 2021Article
- Impact of pneumococcal conjugate vaccine on invasive pneumococcal disease in children under 5 years of age in the Czech Republic.PloS one · 2021Article
- Differential IL-17A response to S. pneumoniae in adenoid tissue of children with sleep disordered breathing and otitis media with effusion.Scientific reports · 2019Article
- Article
- IL-17A expression in the adenoid tissue from children with sleep disordered breathing and its association with pneumococcal carriage.Scientific reports · 2018Article
- Assessment of Postvaccine Immunity againstJournal of immunology research · 2018Article
- Streptococcus pneumoniae antimicrobial resistance decreased in the Helsinki Metropolitan Area after routine 10-valent pneumococcal conjugate vaccination of infants in Finland.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- External Quality Assurance for Laboratory Identification and Capsular Typing of Streptococcus pneumoniae.Scientific reports · 2017Article
- Sequencing of the variable region ofOpen biology · 2017Article
- Improved Differentiation of Streptococcus pneumoniae and Other S. mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm.Journal of clinical microbiology · 2017Article
- Comparison of sequential multiplex PCR, sequetyping and whole genome sequencing for serotyping of Streptococcus pneumoniae.PloS one · 2017Article
- Antimicrobial Resistant Pattern and Capsular Typing of Streptococcus Pneumoniae Isolated from Children in Sistan -Baluchestan.Maedica · 2016Article
- Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.Journal of clinical microbiology · 2016Article
- Etiology of parapneumonic effusion and pleural empyema in children. The role of conventional and molecular microbiological tests.Respiratory medicine · 2016Observational
- Serotypes and genotypes of Streptococcus pneumoniae isolates from Trinidad and Tobago.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2016Article
- Article
- Molecular epidemiology of pneumococcal isolates from children in China.Saudi medical journal · 2016Article
- The PneuCarriage Project: A Multi-Centre Comparative Study to Identify the Best Serotyping Methods for Examining Pneumococcal Carriage in Vaccine Evaluation Studies.PLoS medicine · 2015Article
- Characterization of 19A-like 19F pneumococcal isolates from Papua New Guinea and Fiji.New microbes and new infections · 2015Article
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6 authors.
Funding
Abstract
All currently available vaccines against Streptococcus pneumoniae are based on selections of the over 90 different serotypes, which underlines the importance of serotyping for surveillance and vaccine efficacy monitoring. In this study, we modified and validated a PCR-based scheme for deducing the serotypes of the invasive pneumococci isolated in Finland. For validation, 170 isolates were serotyped using the new protocol with six sequential multiplex PCRs for the deduction of serotypes, supplemented with Quellung testing when needed. The results were compared with those obtained by traditional serotyping methods. We found that 98.8% (168/170) of the isolates were correctly serotyped by the new protocol. Subsequently, the scheme was taken into regular use for serotyping the invasive pneumococci isolated in Finland for serotype-specific surveillance purposes and has been applied in the serotyping of more than 1,500 invasive isolates so far. The sequential multiplex PCRs (mPCRs) have given a result for over 99% of the isolates and allowed us to both handle samples in bulk and noticeably reduce the cost of reagents. While serotyping primarily by PCR is precise and effective, Quellung testing remains the most reliable way to discover possible discrepancies between the DNA deduced and the phenotypic serotype of an isolate. Since implementing the protocol for regular use, two serotype 19F PCR-positive isolates were found to be serotype 19A by the Quellung reaction. While a rare occurrence, this is an important observation, which prompted a revision of our serotyping protocol to prevent possible underreporting of serotype 19A, a potential replacement serotype following large-scale vaccination.
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