ArticleJournal of assisted reproduction and genetics2012
Routine cryopreservation of spermatozoa is safe--evidence from the DNA methylation pattern of nine spermatozoa genes.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Impact of Cryopreservation Extenders on Epigenetic Changes in Bull Sperm: H19 and MEG3 Methylation.Veterinary medicine and science · 2025Article
- Initial motility and vitality predict the semen quality after long-term cryostorage, even in patients with restricted ejaculate parameters.Andrology · 2025Article
- Review
- Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa.Scientific reports · 2023Article
- Cryopreservation of Ovarian and Testicular Tissue and the Influence on Epigenetic Pattern.International journal of molecular sciences · 2023Review
- Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm.International journal of molecular sciences · 2023Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes.Clinical epigenetics · 2022Review
- Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring.Journal of assisted reproduction and genetics · 2022Review
- Effects of Cryopreservation on Sperm with Cryodiluent in Viviparous Black Rockfish (International journal of molecular sciences · 2022Article
- Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common CarpInternational journal of molecular sciences · 2021Article
- Chilling without regrets: Deciphering the effects of cryopreservation on the epigenetic properties of frozen cells will benefit the applications of cryo-technology.EMBO reports · 2016Article
- Nerve growth factor in human semen: Effect of nerve growth factor on the normozoospermic men during cryopreservation process.Iranian journal of basic medical sciences · 2015Article
- Article
- Genetic and Epigenetic Changes After Spermatogonial Stem Cell Culture and Transplantation.EJIFCC · 2014Article
- Update on techniques for cryopreservation of human spermatozoa.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeAssess short- and mid-term impact of cryopreservation on DNA methylation status of different genes in spermatozoa.
methodsSemen samples from 10 healthy normozoospermic men were collected at the Department of Clinical Andrology of the Centre of Reproductive Medicine and Andrology (Muenster, Germany). Each was divided into four equal aliquots: 1) untreated, 2) diluted in cryoprotectant, 3) short term (2 days) cryopreserved and 4) mid term (4 weeks) cryopreserved. Samples were "swim-up" purified prior to analysis. DNA fragmentation was measured using comet assay and Flow cytometric evaluation with Acridine Orange (FCEAO). The degree of methylation of nine genes was determined by bisulfite pyrosequencing of genomic DNA. RESULT(S): Analysis of three maternally imprinted genes (LIT1, SNRPN, MEST), two paternally imprinted genes (MEG3, H19), two repetitive elements (ALU, LINE1), one spermatogenesis-specific gene (VASA) and one gene associated with male infertility (MTHFR) in semen samples demonstrated no alteration in methylation pattern regardless of duration of cryopreservation. CONCLUSION(S): The lack of any changes in the sub-fraction of the genome examined in our study, implies that sperm DNA methylation is unaffected by cryopreservation and suggests that this daily clinical routine is safe in terms of DNA methylation.
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