ArticleActa physiologica (Oxford, England)2013
Alteration in TRPV1 and Muscarinic (M3) receptor expression and function in idiopathic overactive bladder urothelial cells.
Article in Acta physiologica (Oxford, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 46 citations in OpenAlex.
- Genetic variants and expression changes in urgency urinary incontinence: A systematic review.Neurourology and urodynamics · 2020Pooled it
- Pooled it
- Role and mechanism of Roux-en-Y gastric bypass in the treatment of diabetic urinary bladder hyperactivity by reducing TRPV1 and P2X3.World journal of diabetes · 2025Article
- The Efficacy of an Active Medicinal Alkaloid, Berbamine, in Reducing Overactive Bladder Symptoms in a Retinyl Acetate-Induced Model.Biomolecules · 2025Article
- A high salt diet impairs the bladder epithelial barrier and activates the NLRP3 and NF‑κB signaling pathways to induce an overactive bladderExperimental and therapeutic medicine · 2024Article
- Nerve Growth Factor and Brain-Derived Neurotrophic Factor as Potential Biomarkers of Mirabegron Efficacy in Patients with Overactive Bladder Syndrome.International urogynecology journal · 2024Article
- Review
- Overactive bladder syndrome symptoms in youth with abdominal pain-associated disorders of gut-brain interaction.Scientific reports · 2023Article
- Clinical Observation of the Correlation Between Overactive Bladder and Atopic Constitution in Children.Frontiers in pediatrics · 2021Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Development of stress-induced bladder insufficiency requires functional TRPV1 channels.American journal of physiology. Renal physiology · 2018Article
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- 3-Iodothyronamine, a Novel Endogenous Modulator of Transient Receptor Potential Melastatin 8?Frontiers in endocrinology · 2017Review
- BDNF-ERK1/2 signaling pathway in ketamine-associated lower urinary tract symptoms.International urology and nephrology · 2016Article
- Capsaicin: Current Understanding of Its Mechanisms and Therapy of Pain and Other Pre-Clinical and Clinical Uses.Molecules (Basel, Switzerland) · 2016Review
- OnabotulinumtoxinA Treatment for Overactive Bladder in the Elderly: Practical Points and Future Prospects.Drugs & aging · 2016Review
- Effect of botulinum toxin A on urothelial-release of ATP and expression of SNARE targets within the urothelium.Neurourology and urodynamics · 2015Article
- Article
- Transient receptor potential cation channels in visceral sensory pathways.British journal of pharmacology · 2014Review
- TRP channels in lower urinary tract dysfunction.British journal of pharmacology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
aimTo examine function of both cholinergic (muscarinic) and TRPV1 receptors in human bladder urothelial (HBUC) from non-neurogenic overactive bladder (OAB) patients as compared to control subjects.
methodsPrimary HBUC cultures were derived from cystoscopic biopsies from OAB and control subjects. Muscarinic and TRPV1 function was assessed by acetylcholine (5 μm) or capsaicin (0.5 μm) evoked ATP release, measured by luciferase assay. Overall, expression of TRPV1 and muscarinic M3 receptors in bladder urothelial cells was accomplished using western immunoblotting.
resultsOur findings revealed that the response to acetylcholine in OAB HBUC cultures (which was blocked by the nonselective muscarinic antagonist, atropine methyl nitrate or AMN) was not significantly different than from controls. The acetylcholine M3 receptor was slightly decreased as compared to control. In contrast, OAB HBUC cultures exhibited a capsaicin hypersensitivity and augmented release of ATP (3.2 fold higher), which was blocked by the antagonist capsazepine. The increase in capsaicin sensitivity correlated with increased urothelial TRPV1 expression.
conclusionThough characterized in a small number of subjects, augmented release of urothelial-derived transmitters such as ATP could 'amplify' signalling between and within urothelial cells and nearby afferent nerves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.