ArticleBMC microbiology2012
Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- The carbon starvation-inducible lipoprotein (Slp) influences differential adherence of Escherichia coli O157:H7 at the bovine rectoanal junction.PLoS pathogens · 2026Article
- Distinct Tissue Localization and Transcriptional Profiles of Shiga Toxin-ProducingInternational journal of microbiology · 2026Article
- Investigating the adherence factors ofMicrobiology spectrum · 2024Article
- Phenotypic and genomic comparison of three human outbreak and one cattle-associated Shiga toxin-producingMicrobiology spectrum · 2024Article
- Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-ProducingMicroorganisms · 2023Article
- Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle.Scientific reports · 2021Article
- Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.International journal of medical microbiology : IJMM · 2021Article
- Nonfimbrial Adhesin Mutants Reveal DivergentInternational journal of microbiology · 2021Article
- An Overview of the Elusive Passenger in the Gastrointestinal Tract of Cattle: The Shiga Toxin ProducingMicroorganisms · 2020Review
- Differential expression of the virulence geneHeliyon · 2020Article
- SupershedInternational journal of microbiology · 2020Article
- Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome.PloS one · 2018Article
- Immune Response in Calves Vaccinated with Type Three Secretion System Antigens and Shiga Toxin 2B Subunit of Escherichia coli O157:H7.PloS one · 2017Article
- Curli Temper Adherence of Escherichia coli O157:H7 to Squamous Epithelial Cells from the Bovine Recto-Anal Junction in a Strain-Dependent Manner.Applied and environmental microbiology · 2017Article
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- Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes.PloS one · 2015Article
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- Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns.BMC veterinary research · 2013Article
- Growth media simulating ileal and colonic environments affect the intracellular proteome and carbon fluxes of enterohemorrhagic Escherichia coli O157:H7 strain EDL933.Applied and environmental microbiology · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIn this study, we present evidence that proteins encoded by the Locus of Enterocyte Effacement (LEE), considered critical for Escherichia coli O157 (O157) adherence to follicle-associated epithelial (FAE) cells at the bovine recto-anal junction (RAJ), do not appear to contribute to O157 adherence to squamous epithelial (RSE) cells also constituting this primary site of O157 colonization in cattle.
resultsAntisera targeting intimin-γ, the primary O157 adhesin, and other essential LEE proteins failed to block O157 adherence to RSE cells, when this pathogen was grown in DMEM, a culture medium that enhances expression of LEE proteins. In addition, RSE adherence of a DMEM-grown-O157 mutant lacking the intimin protein was comparable to that seen with its wild-type parent O157 strain grown in the same media. These adherence patterns were in complete contrast to that observed with HEp-2 cells (the adherence to which is mediated by intimin-γ), assayed under same conditions. This suggested that proteins other than intimin-γ that contribute to adherence to RSE cells are expressed by this pathogen during growth in DMEM. To identify such proteins, we defined the proteome of DMEM-grown-O157 (DMEM-proteome). GeLC-MS/MS revealed that the O157 DMEM-proteome comprised 684 proteins including several components of the cattle and human O157 immunome, orthologs of adhesins, hypothetical secreted and outer membrane proteins, in addition to the known virulence and LEE proteins. Bioinformatics-based analysis of the components of the O157 DMEM proteome revealed several new O157-specific proteins with adhesin potential.
conclusionProteins other than LEE and intimin-γ proteins are involved in O157 adherence to RSE cells at the bovine RAJ. Such proteins, with adhesin potential, are expressed by this human pathogen during growth in DMEM. Ongoing experiments to evaluate their role in RSE adherence should provide both valuable insights into the O157-RSE interactions and new targets for more efficacious anti-adhesion O157 vaccines.
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