ArticleMolecular vision2012
Experimental murine myopia induces collagen type Iα1 (COL1A1) DNA methylation and altered COL1A1 messenger RNA expression in sclera.
Article in Molecular vision, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Intermittent myopic visual exposure triggers myopia progression via H3K27me3.Genome medicine · 2026Article
- An ovary-intact postmenopausal HFpEF mouse model; menopause is more than just estrogen deficiency.American journal of physiology. Heart and circulatory physiology · 2025Article
- Research Progress of Epigenetic Modifications in Myopia.International journal of medical sciences · 2025Review
- The potential of brimonidine for myopia treatment: Targeting MMP-2 to regulate choroidal thickness and control eye growth.Heliyon · 2024Article
- Establishment of a novel ER-stress induced myopia model in mice.Eye and vision (London, England) · 2023Article
- Association of Polymorphisms in ZFHX1B and PAX6 With Anisometropia in Chinese Children: The Hong Kong Children Eye Genetics Study.Investigative ophthalmology & visual science · 2023Article
- Epitranscriptomic investigation of myopia-associated RNA editing in the retina.Frontiers in neuroscience · 2023Article
- Single-cell RNA sequencing reveals a unique pericyte type associated with capillary dysfunction.Theranostics · 2023Article
- miR-328-3p Affects Axial Length Via Multiple Routes and Anti-miR-328-3p Possesses a Potential to Control Myopia Progression.Investigative ophthalmology & visual science · 2022Article
- Decreased Levels of DNA Methylation in the PCDHA Gene Cluster as a Risk Factor for Early-Onset High Myopia in Young Children.Investigative ophthalmology & visual science · 2022Article
- Intravitreal brimonidine inhibits form-deprivation myopia in guinea pigs.Eye and vision (London, England) · 2021Article
- Genipin inhibits the scleral expression of miR-29 and MMP2 and promotes COL1A1 expression in myopic eyes of guinea pigs.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020Article
- RNA sequencing analysis of long non-coding RNA expression in ocular posterior poles of guinea pig myopia models.Molecular vision · 2020Article
- IMI - Myopia Genetics Report.Investigative ophthalmology & visual science · 2019Review
- In-utero epigenetic factors are associated with early-onset myopia in young children.PloS one · 2019Article
- Genome-wide DNA hypermethylation and homocysteine increase a risk for myopia.International journal of ophthalmology · 2019Article
- Myopia genetics in genome-wide association and post-genome-wide association study era.International journal of ophthalmology · 2019Review
- Insight into the molecular genetics of myopia.Molecular vision · 2017Review
- Altered Refractive Development in Mice With Reduced Levels of Retinal Dopamine.Investigative ophthalmology & visual science · 2016Article
- Genetic association of COL1A1 polymorphisms with high myopia in Asian population: a Meta-analysis.International journal of ophthalmology · 2016Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate whether myopia development is associated with changes of scleral DNA methylation in cytosine-phosphate-guanine (CpG) sites in the collagen 1A1 (COL1A1) promoter and messenger RNA (mRNA) levels following murine form deprivation myopia.
methodsFifty-seven C57BL/6 mice (postnatal day 23) were randomly assigned to four groups: (1) monocular form deprivation (MD) in which a diffuser lens was placed over one eye for 28 days; (2) normal controls without MD; (3) MD recovery in which the diffuser lens was removed for seven days; and (4) MD recovery normal controls. The DNA methylation pattern in COL1A1 promoter and exon 1 was determined by bisulfite DNA sequencing, and the COL1A1 mRNA level in sclera was determined by quantitative PCR.
resultsMD was found to induce myopia in the treated eyes. Six CpG sites in the promoter and exon 1 region of COL1A1 were methylated with significantly higher frequency in the treated eyes than normal control eyes (p<0.05), with CpG island methylation in MD-contralateral eyes being intermediate. Consistent with the CpG methylation, scleral COL1A1 mRNA was reduced by 57% in the MD-treated eyes compared to normal controls (p<0.05). After seven days of MD recovery, CpG methylation was significantly reduced (p=0.01). The methylation patterns returned to near normal level in five CpG sites, but the sixth was hypomethylated compared to normal controls.
conclusionsIn parallel with the development of myopia and the reduced COL1A1 mRNA, the frequency of methylation in CpG sites of the COL1A1 promoter/exon 1 increased during MD and returned to near normal during recovery. Thus, hypermethylation of CpG sites in the promoter/exon 1 of COL1A1 may underlie reduced collagen synthesis at the transcriptional level in myopic scleras.
Indexed as
Identifiers
22690110PMC3369898W2163166807What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.