ArticleWorld journal of gastroenterology2012
High dose glargine alters the expression profiles of microRNAs in pancreatic cancer cells.
Article in World journal of gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- In Vivo Stable Allogenic Cartilage Regeneration in a Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes.Advanced healthcare materials · 2023Article
- Survival differences and associated molecular signatures of DNMT3A-mutant acute myeloid leukemia patients.Scientific reports · 2020Article
- Insulin glargine affects the expression ofIranian journal of basic medical sciences · 2018Article
- Non-coding RNAs in pancreatic cancer: challenges and opportunities for clinical application.Cellular oncology (Dordrecht, Netherlands) · 2016Review
- Development of a circulating miRNA assay to monitor tumor burden: From mouse to man.Molecular oncology · 2016Article
- Biological effects of low-dose-rate irradiation of pancreatic carcinoma cells in vitro using 125I seeds.World journal of gastroenterology · 2015Article
- Cancer biology in diabetes.Journal of diabetes investigation · 2014Review
- Role of the ERas gene in gastric cancer cells.Oncology reports · 2013Article
- Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in cervical cancer.Virology journal · 2013Article
- The links between insulin resistance, diabetes, and cancer.Current diabetes reports · 2013Review
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effect of high dose glargine on the expression profiles of microRNAs in human pancreatic cancer cells.
methodsReal-time polymerase chain reaction array (RT-PCR) was applied to investigate miRNAs differentially expressed in Sw1990 cells treated with or without 100 IU/L glargine. Stem-loop RT-PCR was used to confirm the results of the array assay in Sw1990 and Panc-1 cells. The effects of miR-95 on cell growth, apoptosis, invasion and migration abilities were respectively examined by CCK8 assay, apoptosis assay, Matrigel invasion and migration assay in Sw1990 and Panc-1 cells. Nude mice xenograft models with Sw1990 cells were built to investigate pancreatic cancer growth in vivo after transfection by the lentivirus pGLV3-GFP- miR-95.
resultsTen miRNAs were significantly up-regulated and 2 miRNAs down-regulated in glargine treated Sw1990 cells when compared with non-treated cells (2.48-fold changes on average, P < 0.01). miR-95, miR-134 and miR-34c-3p are the top three miRNAs regulated by glargine (3.65-fold, 2.67-fold and 2.60-fold changes respectively, P < 0.01) in Sw1990 cells. Stem-loop RT-PCR confirmed that high dose glargine up-regulated the expression of miR-95 and miR-134 in both Sw1990 and Panc-1 cells. The most obvious change is the apparent increase of miR-95. Forced expression of miR-95 significantly increased cell proliferation (Sw1990: 2.510 ± 0.129 vs 2.305 ± 0.187, P < 0.05; Panc-1: 2.439 ± 0.211 vs 2.264 ± 0.117, P < 0.05), invasion (Sw1990: 67.90 ± 12.33 vs 47.30 ± 5.89, P < 0.01; Panc-1: 37.80 ± 8.93 vs 30.20 ± 5.14, P < 0.01), migration (Sw1990: 101 ± 6.00 vs 51.20 ± 8.34, P < 0.01; Panc-1: 91.80 ± 9.22 vs 81.50 ± 7.47, P < 0.01) and inhibited cell apoptosis (Sw1990: 22.05% ± 1.92% vs 40.32% ± 1.93%, P < 0.05; Panc-1: 20.17% ± 0.85% vs 45.60% ± 1.43%, P < 0.05) when compared with paired negative controls, whereas knockdown of miR-95 obtained the opposite effect. Nude mice xenograft models confirmed that miR-95 promoted the growth of pancreatic cancer in vivo when compared with negative control (tumor volume: 373.82 ± 23.67 mL vs 219.69 ± 17.82 mL, P < 0.05).
conclusionThese observations suggested that modulation of miRNA expression may be an important mechanism underlying the biological effects of glargine.
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