ArticleProceedings of the National Academy of Sciences of the United States of America2012
Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Mitotic chromosome condensation resets chromatin to safeguard transcriptional homeostasis during interphase.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell Cycle-Dependent Transcription: The Cyclin Dependent Kinase Cdk1 Is a Direct Regulator of Basal Transcription Machineries.International journal of molecular sciences · 2022Review
- Review
- GScience (New York, N.Y.) · 2021Article
- Coupling Between Cell Cycle Progression and the Nuclear RNA Polymerases System.Frontiers in molecular biosciences · 2021Review
- UniProt-Related Documents (UniReD): assisting wet lab biologists in their quest on finding novel counterparts in a protein network.NAR genomics and bioinformatics · 2020Article
- The cell-cycle transcriptional network generates and transmits a pulse of transcription once each cell cycle.Cell cycle (Georgetown, Tex.) · 2019Article
- Coupling of RNA polymerase III assembly to cell cycle progression in Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2019Article
- Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner.Nature chemical biology · 2019Article
- Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity.Nucleic acids research · 2018Article
- Article
- Single-cell RNA sequencing reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress.PLoS biology · 2017Article
- OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc.Oncogene · 2017Article
- Mapping the Synthetic Dosage Lethality Network ofG3 (Bethesda, Md.) · 2017Article
- Cdc15 Phosphorylates the C-terminal Domain of RNA Polymerase II for Transcription during Mitosis.The Journal of biological chemistry · 2017Article
- TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Clb/Cdk1-mediated regulation of Fkh2 synchronizesNPJ systems biology and applications · 2017Article
- Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.Molecular cell · 2016Article
- Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes.Genome research · 2015Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The cyclin-dependent kinase Cdc28 is the master regulator of the cell cycle in Saccharomyces cerevisiae. Cdc28 initiates the cell cycle by activating cell-cycle-specific transcription factors that switch on a transcriptional program during late G1 phase. Cdc28 also has a cell-cycle-independent, direct function in regulating basal transcription, which does not require its catalytic activity. However, the exact role of Cdc28 in basal transcription remains poorly understood, and a function for its kinase activity has not been fully explored. Here we show that the catalytic activity of Cdc28 is important for basal transcription. Using a chemical-genetic screen for mutants that specifically require the kinase activity of Cdc28 for viability, we identified a plethora of basal transcription factors. In particular, CDC28 interacts genetically with genes encoding kinases that phosphorylate the C-terminal domain of RNA polymerase II, such as KIN28. ChIP followed by high-throughput sequencing (ChIP-seq) revealed that Cdc28 localizes to at least 200 genes, primarily with functions in cellular homeostasis, such as the plasma membrane proton pump PMA1. Transcription of PMA1 peaks early in the cell cycle, even though the promoter sequences of PMA1 (as well as the other Cdc28-enriched ORFs) lack cell-cycle elements, and PMA1 does not recruit Swi4/6-dependent cell-cycle box-binding factor/MluI cell-cycle box binding factor complexes. Finally, we found that recruitment of Cdc28 and Kin28 to PMA1 is mutually dependent and that the activity of both kinases is required for full phosphorylation of C-terminal domain-Ser5, for efficient transcription, and for mRNA capping. Our results reveal a mechanism of cell-cycle-dependent regulation of basal transcription.
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