ArticleNucleic acids research2012
MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- A Precise TaqMan-Based Real-Time qPCR Assay for Detecting and Quantifying Blackberry Chlorotic Ringspot Virus, Blueberry Shock Virus, and Plum Pox Virus in Fruit Tree Seedlings.The plant pathology journal · 2025Article
- Examining hybridization-based LC-MS methodologies for the bioanalysis of siRNA analytes.Bioanalysis · 2025Article
- Impact of MTHFR gene polymorphism on the outcome of methotrexate treatment in a sample of Iraqi rheumatoid arthritis patients.Scientific reports · 2024Article
- A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.Forestry research · 2024Article
- Curcumin supplementation increases longevity and antioxidant capacity inFrontiers in pharmacology · 2023Article
- High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants.Scientific reports · 2022Article
- Development and Validation of a New TaqMan Real-Time PCR for the Detection ofMicroorganisms · 2022Article
- A targeted amplicon sequencing panel to simultaneously identify mosquito species and Plasmodium presence across the entire Anopheles genus.Molecular ecology resources · 2022Article
- Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation.Cell reports · 2021Article
- Mikan Genome Database (MiGD): integrated database of genome annotation, genomic diversity, and CAPS marker information for mandarin molecular breeding.Breeding science · 2020Article
- Article
- Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae.Frontiers in microbiology · 2020Article
- TASUKE+: a web-based platform for exploring genome-wide association studies results and large-scale resequencing data.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Article
- MRPrimerW2: an enhanced tool for rapid design of valid high-quality primers with multiple search modes for qPCR experiments.Nucleic acids research · 2019Article
- MFEprimer-3.0: quality control for PCR primers.Nucleic acids research · 2019Article
- Ranolazine prevents pressure overload-induced cardiac hypertrophy and heart failure by restoring aberrant NaJournal of cellular physiology · 2019Article
- PrimerROC: accurate condition-independent dimer prediction using ROC analysis.Scientific reports · 2019Article
- In-silico Design of DNA Oligonucleotides: Challenges and Approaches.Computational and structural biotechnology journal · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evaluating the specificity of polymerase chain reaction (PCR) primers is an essential step in PCR primer design. The MFEprimer-2.0 server allows users to check primer specificity against genomic DNA and messenger RNA/complementary DNA sequence databases quickly and easily. MFEprimer-2.0 uses a k-mer index algorithm to accelerate the search process for primer binding sites and uses thermodynamics to evaluate binding stability between each primer and its DNA template. Several important characteristics, such as the sequence, melting temperature and size of each amplicon, either specific or non-specific, are reported on the results page. Based on these characteristics and the user-friendly output, users can readily draw conclusions about the specificity of PCR primers. Analyses for degenerate primers and multiple PCR primers are also supported in MFEprimer-2.0. In addition, the databases supported by MFEprimer-2.0 are comprehensive, and custom databases can also be supported on request. The MFEprimer-2.0 server does not require a login and is freely available at http://biocompute.bmi.ac.cn/CZlab/MFEprimer-2.0. More over, the MFEprimer-2.0 command-line version and local server version are open source and can be downloaded at https://github.com/quwubin/MFEprimer/wiki/Manual/.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.