ArticleThe Journal of biological chemistry2012
c-Src but not Fyn promotes proper spindle orientation in early prometaphase.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 57 citations in OpenAlex.
- The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1.The Journal of biological chemistry · 2026Article
- Targeting YES1 Disrupts Mitotic Fidelity and Potentiates the Response to Taxanes in Triple-Negative Breast Cancer.Cancer research · 2024Article
- Src inhibition induces mitotic arrest associated with chromosomal passenger complex.Cell and tissue research · 2023Article
- SRC Kinase-Mediated Tyrosine Phosphorylation of TUBB3 Regulates Its Stability and Mitotic Spindle Dynamics in Prostate Cancer Cells.Pharmaceutics · 2022Article
- Par-3 family proteins in cell polarity & adhesion.The FEBS journal · 2022Review
- Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Combination Treatment of OSI-906 with Aurora B Inhibitor Reduces Cell Viability via Cyclin B1 Degradation-Induced Mitotic Slippage.International journal of molecular sciences · 2021Article
- Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.Scientific reports · 2021Article
- Depletion of Csk preferentially reduces the protein level of LynA in a Cbl-dependent manner in cancer cells.Scientific reports · 2020Article
- Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition.Life science alliance · 2020Article
- Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells.Nature communications · 2019Article
- Emerging role for SRC family kinases in junction dynamics during spermatogenesis.Reproduction (Cambridge, England) · 2019Review
- Inhibitors of the VEGF Receptor Suppress HeLa S3 Cell Proliferation via Misalignment of Chromosomes and Rotation of the Mitotic Spindle, Causing a Delay in M-Phase Progression.International journal of molecular sciences · 2018Article
- The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1.The Journal of biological chemistry · 2018Article
- Src activation decouples cell division orientation from cell geometry in mammalian cells.Biomaterials · 2018Article
- Air-drying of cells enables visualization of antiparallel microtubule overlaps in the spindle midzone.MethodsX · 2018Article
- Palladin is a novel microtubule-associated protein responsible for spindle orientation.Scientific reports · 2017Article
- Src family kinase phosphorylation of the motor domain of the human kinesin-5, Eg5.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Antitumor Effect of KX-01 through Inhibiting Src Family Kinases and Mitosis.Cancer research and treatment · 2017Article
- Tyr(less) kinase signaling during mitosis.Cell cycle (Georgetown, Tex.) · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Src family tyrosine kinases (SFKs) participate in mitotic signal transduction events, including mitotic entry, cleavage furrow ingression, and cytokinesis abscission. Although SFKs have been shown to associate with the mitotic spindle, the role of SFKs in mitotic spindle formation remains unclear. Here, we show that c-Src promotes proper spindle orientation in early prometaphase. Src localizes close to spindle poles in a manner independent of Src kinase activity. Three-dimensional analyses showed that Src inhibition induced spindle misorientation, exhibiting a tilting spindle in early prometaphase. Spindle misorientation is frequently seen in SYF cells, which harbor triple knock-out mutations of c-Src, c-Yes, and Fyn, and reintroduction of c-Src but not Fyn into SYF cells rescued spindle misorientation. Spindle misorientation was also observed upon Src inhibition under conditions in which Aurora B was inhibited. Inducible expression of c-Src promoted a properly oriented bipolar spindle, which was suppressed by Src inhibition. Aster formation was severely inhibited in SYF cells upon Aurora B inhibition, which was rescued by reintroduction of c-Src into SYF cells. Furthermore, reintroduction of c-Src facilitated microtubule regrowth from cold-induced depolymerization and accelerated M phase progression. These results suggest that c-Src is involved in spindle orientation through centrosome-mediated aster formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.