ArticleMolecular and cellular biology2012
AMPK regulates mitotic spindle orientation through phosphorylation of myosin regulatory light chain.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 69 citations in OpenAlex.
- Nuclear localization of MTHFD2 is required for correct mitosis progression.Nature communications · 2024Article
- Therapeutic modulation of ROCK overcomes metabolic adaptation of cancer cells to OXPHOS inhibition and drives synergistic anti-tumor activity.bioRxiv : the preprint server for biology · 2024Article
- E3 Ubiquitin LigaseInternational journal of molecular sciences · 2024Article
- COX6C expression driven by copy amplification of 8q22.2 regulates cell proliferation via mediation of mitosis by ROS-AMPK signaling in lung adenocarcinoma.Cell death & disease · 2024Article
- Cigarette smoke condensate induces centrosome clustering in normal lung epithelial cells.Cancer medicine · 2023Article
- PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis.Cell structure and function · 2022Article
- Understanding the underlying mechanisms governing spindle orientation: How far are we from there?Journal of cellular and molecular medicine · 2022Review
- AMPKα2 activation by an energy-independent signal ensures chromosomal stability during mitosis.iScience · 2021Article
- Mitotic Spindle Apparatus Abnormalities in Chronic Obstructive Pulmonary Disease Cells: A Potential Pathway to Lung Cancer.Cancer prevention research (Philadelphia, Pa.) · 2020Article
- ULK1 inhibitor induces spindle microtubule disorganization and inhibits phosphorylation of Ser10 of histone H3.FEBS open bio · 2020Article
- Loss of AMPKalpha1 Triggers Centrosome Amplification via PLK4 Upregulation in Mouse Embryonic Fibroblasts.International journal of molecular sciences · 2020Article
- Girdin is a component of the lateral polarity protein network restricting cell dissemination.PLoS genetics · 2020Article
- Insight on Transcriptional Regulation of the Energy Sensing AMPK and Biosynthetic mTOR Pathway Genes.Frontiers in cell and developmental biology · 2020Review
- Cyclin-dependent kinase 1-mediated AMPK phosphorylation regulates chromosome alignment and mitotic progression.Journal of cell science · 2019Article
- Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells.Nature communications · 2019Article
- Mechanisms linking mechanotransduction and cell metabolism.Current opinion in cell biology · 2018Review
- LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Kinases Involved in Both Autophagy and Mitosis.International journal of molecular sciences · 2017Review
- The acidic microenvironment as a possible niche of dormant tumor cells.Cellular and molecular life sciences : CMLS · 2017Review
- Entosis Is Induced by Glucose Starvation.Cell reports · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The proper orientation of the mitotic spindle is essential for mitosis; however, how these events unfold at the molecular level is not well understood. AMP-activated protein kinase (AMPK) regulates energy homeostasis in eukaryotes, and AMPK-null Drosophila mutants have spindle defects. We show that threonine(172) phosphorylated AMPK localizes to the mitotic spindle poles and increases when cells enter mitosis. AMPK depletion causes a mitotic delay with misoriented spindles relative to the normal division plane and a reduced number and length of astral microtubules. AMPK-depleted cells contain mitotic actin bundles, which prevent astral microtubule-actin cortex attachments. Since myosin regulatory light chain (MRLC) is an AMPK downstream target and mediates actin function, we investigated whether AMPK signals through MRLC to control spindle orientation. Mitotic levels of serine(19) phosphorylated MRLC (pMRLC(ser19)) and spindle pole-associated pMRLC(ser19) are abolished when AMPK function is compromised, indicating that AMPK is essential for pMRLC(ser19) spindle pole activity. Phosphorylation of AMPK and MRLC in the mitotic spindle is dependent upon calcium/calmodulin-dependent protein kinase kinase (CamKK) activity in LKB1-deficient cells, suggesting that CamKK regulates this pathway when LKB1 function is compromised. Taken together, these data indicate that AMPK mediates spindle pole-associated pMRLC(ser19) to control spindle orientation via regulation of actin cortex-astral microtubule attachments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.