ArticleBiomaterials2012
Hyaluronic acid-based hydrogels functionalized with heparin that support controlled release of bioactive BMP-2.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 186 citations in OpenAlex.
- Heparin-Based Biomaterials for Sustained Release of Growth Factors for Bone Tissue Engineering and Regeneration.Journal of functional biomaterials · 2026Review
- Engineering a BMP2-risedronate complex with sustained release for osteoporosis therapy.Archives of pharmacal research · 2025Article
- Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice.Scientific reports · 2025Article
- 3D-Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical-Size Bone Defect.Advanced healthcare materials · 2023Article
- Biomimetic Inspired Hydrogels for Regenerative Vertebral Body Stenting.Current osteoporosis reports · 2023Review
- Recent Progress in Hyaluronic-Acid-Based Hydrogels for Bone Tissue Engineering.Gels (Basel, Switzerland) · 2023Review
- Multiscale design of stiffening and ROS scavenging hydrogels for the augmentation of mandibular bone regeneration.Bioactive materials · 2023Article
- Investigations into the effects of scaffold microstructure on slow-release system with bioactive factors for bone repair.Frontiers in bioengineering and biotechnology · 2023Review
- Carrier systems for bone morphogenetic proteins: An overview of biomaterials used for dentoalveolar and maxillofacial bone regeneration.The Japanese dental science review · 2022Review
- Locally Delivered Metabolite Derivative Promotes Bone Regeneration in Aged Mice.ACS applied bio materials · 2022Article
- Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in aniScience · 2022Article
- Bioengineering Approaches for Delivering Growth Factors: A Focus on Bone and Cartilage Regeneration.Bioengineering (Basel, Switzerland) · 2022Review
- Fabrication of gentamicin loaded Col-I/HA multilayers modified titanium coatings for prevention of implant infection.Frontiers in chemistry · 2022Article
- The Auxiliary Role of Heparin in Bone Regeneration and its Application in Bone Substitute Materials.Frontiers in bioengineering and biotechnology · 2022Review
- Recent Progress on Polysaccharide-Based Hydrogels for Controlled Delivery of Therapeutic Biomolecules.ACS biomaterials science & engineering · 2021Review
- Methods of Topical Administration of Drugs and Biological Active Substances for Dental Implants-A Narrative Review.Antibiotics (Basel, Switzerland) · 2021Article
- Bone Morphogenetic Proteins, Carriers, and Animal Models in the Development of Novel Bone Regenerative Therapies.Materials (Basel, Switzerland) · 2021Review
- Cyclodextrin-Based Supramolecular Complexes of Osteoinductive Agents for Dental Tissue Regeneration.Pharmaceutics · 2021Review
- The 3-Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Enzymatic Synthesis of Glycans and Glycoconjugates.Advances in biochemical engineering/biotechnology · 2021Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive factor, yet its clinical use is limited by a short biological half-life, rapid local clearance and propensity for side effects. Heparin (HP), a highly sulfated glycosaminoglycan (GAG) that avidly binds BMP-2, has inherent biological properties that may circumvent these limitations. Here, we compared hyaluronan-based hydrogels formulated to include heparin (Heprasil™) with similar gels without heparin (Glycosil™) for their ability to deliver bioactive BMP-2 in vitro and in vivo. The osteogenic activity of BMP-2 released from the hydrogels was evaluated by monitoring alkaline phosphatase (ALP) activity and SMAD 1/5/8 phosphorylation in mesenchymal precursor cells. The osteoinductive ability of these hydrogels was determined in a rat ectopic bone model by 2D radiography, 3D μ-CT and histological analyses at 8 weeks post-implantation. Both hydrogels sustain the release of BMP-2. Importantly, the inclusion of a small amount of heparin (0.3% w/w) attenuated release of BMP-2 and sustained its osteogenic activity for up to 28 days. In contrast, hydrogels lacking heparin released more BMP-2 initially but were unable to maintain BMP-2 activity at later time points. Ectopic bone-forming assays using transplanted hydrogels emphasized the therapeutic importance of the initial burst of BMP-2 rather than its long-term osteogenic activity. Thus, tuning the burst release phase of BMP-2 from hydrogels may be advantageous for optimal bone formation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.