Evidence map›Paper›PMID 22687611›Full record

ArticleThe Journal of physiology2012

Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle.

Andrés Hernández, Tomas A Schiffer, Niklas Ivarsson, Arthur J Cheng, Joseph D Bruton, Jon O Lundberg, Eddie Weitzberg, Håkan Westerblad

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers, 14 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
149citing papers in PubMed, 14 pooled it
55.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

149 citing papers in PubMed, 14 syntheses or guidelines pooled it, 307 citations in OpenAlex.

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  7. The Effects of Dietary Nitrate Supplementation on Explosive Exercise Performance: A Systematic Review.International journal of environmental research and public health · 2022
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89 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Andrés HernándezDepartment of Physiology and Pharmacology, Karolinska Institutet, SE-171 77 Stockholm, Sweden. andres.hernandez@ki.se
Tomas A Schiffer
Niklas Ivarsson
Arthur J Cheng
Joseph D Bruton
Jon O Lundberg
Eddie Weitzberg
Håkan Westerblad
Karolinska Institutet · SE

Funding

Mechanisms of Mitochondrial MyopathyF32AR057619 · NIAMS · KAROLINSKA INSTITUTE · PI HERNANDEZ, ANDRES · 2011 to 2013
$155k
NIAMS NIH HHS 1F32AR057619NIAMS NIH HHS F32 AR057619
6 · The paper itself

Abstract

Dietary inorganic nitrate has profound effects on health and physiological responses to exercise. Here, we examined if nitrate, in doses readily achievable via a normal diet, could improve Ca(2+) handling and contractile function using fast- and slow-twitch skeletal muscles from C57bl/6 male mice given 1 mm sodium nitrate in water for 7 days. Age matched controls were provided water without added nitrate. In fast-twitch muscle fibres dissected from nitrate treated mice, myoplasmic free [Ca(2+)] was significantly greater than in Control fibres at stimulation frequencies from 20 to 150 Hz, which resulted in a major increase in contractile force at ≤ 50 Hz. At 100 Hz stimulation, the rate of force development was ∼35% faster in the nitrate group. These changes in nitrate treated mice were accompanied by increased expression of the Ca(2+) handling proteins calsequestrin 1 and the dihydropyridine receptor. No changes in force or calsequestrin 1 and dihydropyridine receptor expression were measured in slow-twitch muscles. In conclusion, these results show a striking effect of nitrate supplementation on intracellular Ca(2+) handling in fast-twitch muscle resulting in increased force production. A new mechanism is revealed by which nitrate can exert effects on muscle function with applications to performance and a potential therapeutic role in conditions with muscle weakness.

Indexed as

AnimalsCalciumCalcium-Binding ProteinsCalcium Channels, L-TypeCalsequestrinDietMaleMiceMice, Inbred C57BLMuscle ContractionMuscle Fibers, Fast-TwitchNitratesRyanodine Receptor Calcium Release ChannelCalciumCalcium-Binding ProteinsCalcium Channels, L-TypeCalsequestrinCasq1 protein, mousecasq2 protein, mouseNitratesRyanodine Receptor Calcium Release Channel

Identifiers

PMID22687611
PMCPMC3547271
OpenAlexW2120006556

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.