Trial reportCellular reprogramming2012
Handmade cloned transgenic piglets expressing the nematode fat-1 gene.
Trial report in Cellular reprogramming, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster.Transgenic research · 2023Article
- Genome Editing and Protein Energy Malnutrition.Advances in experimental medicine and biology · 2023Article
- CRISPR/Cas9-Mediated Specific Integration ofGenes · 2021Article
- Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals.Veterinary sciences · 2021Review
- Efficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9.Scientific reports · 2020Article
- Practical Approaches for Knock-Out Gene Editing in Pigs.Frontiers in genetics · 2020Review
- Co-expression of fat1 and fat2 in transgenic pigs promotes synthesis of polyunsaturated fatty acids.Transgenic research · 2019Article
- A Novel Dietary Source of EPA and DHA: Metabolic Engineering of an Important Freshwater Species-Common Carp by fat1-Transgenesis.Marine biotechnology (New York, N.Y.) · 2019Article
- Livestock 2.0 - genome editing for fitter, healthier, and more productive farmed animals.Genome biology · 2018Review
- Site-Specific Fat-1 Knock-In Enables Significant Decrease of n-6PUFAs/n-3PUFAs Ratio in Pigs.G3 (Bethesda, Md.) · 2018Article
- The role of polyunsaturated fatty acids (n-3 PUFAs) on the pancreatic β-cells and insulin action.Adipocyte · 2018Review
- DNMT 1 maintains hypermethylation of CAG promoter specific region and prevents expression of exogenous gene in fat-1 transgenic sheep.PloS one · 2017Article
- Production of Transgenic Pigs with an Introduced Missense Mutation of the Bone Morphogenetic Protein Receptor Type IB Gene Related to Prolificacy.Asian-Australasian journal of animal sciences · 2016Article
- Using Caenorhabditis elegans to Uncover Conserved Functions of Omega-3 and Omega-6 Fatty Acids.Journal of clinical medicine · 2016Review
- Microalgal Oil Supplementation Has an Anti-Obesity Effect in C57BL/6J Mice Fed a High Fat Diet.Preventive nutrition and food science · 2015Article
- Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos.Cellular reprogramming · 2015Article
- The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production.Scientific reports · 2015Article
- Handmade cloning: recent advances, potential and pitfalls.Journal of animal science and biotechnology · 2015Review
- Double transgenesis of humanized fat1 and fat2 genes promotes omega-3 polyunsaturated fatty acids synthesis in a zebrafish model.Marine biotechnology (New York, N.Y.) · 2014Article
- The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae.Transgenic research · 2014Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established previously to produce transgenic pigs that express the functional nematode fat-1 gene. Codon-optimized mfat-1 was inserted into eukaryotic expression vectors, which were transferred into primary swine donor cells. Reverse transcriptase PCR (RT-PCR), gas chromatography, and chromosome analyses were performed to select donor clones capable of converting n-6 into n-3 fatty acids. Blastocysts derived from the clones that lowered the n-6/n-3 ratio to approximately 1:1 were transferred surgically into the uteri of recipients for transgenic piglets. By HMC, 37% (n=558) of reconstructed embryos developed to the blastocyst stage after 7 days of culture in vitro, with an average cell number of 81±36 (n=14). Three recipients became pregnant after 408 day-6 blastocysts were transferred into four naturally cycling females, and a total of 14 live offspring were produced. The nematode mfat-1 effectively lowered the n-6/n-3 ratio in muscle and major organs of the transgenic pig. Our results will help to establish a reliable procedure and an efficient option in the production of transgenic animals.
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