ArticleCellular reprogramming2012
Human amniotic epithelial cells are reprogrammed more efficiently by induced pluripotency than adult fibroblasts.
Article in Cellular reprogramming, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Application of human amniotic epithelial cells in regenerative medicine: a systematic review.Stem cell research & therapy · 2020Pooled it
- Characterization of amniotic stem cells.Cellular reprogramming · 2014Trial
- Inducing Pluripotency in Somatic Cells: Historical Perspective and Recent Advances.International journal of stem cells · 2024Review
- Putative epithelial-mesenchymal transitions during salamander limb regeneration: Current perspectives and future investigations.Annals of the New York Academy of Sciences · 2024Review
- Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications.International journal of molecular sciences · 2020Review
- Fetal Membrane Epigenetics.Frontiers in physiology · 2020Review
- In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells.Stem cell research & therapy · 2017Article
- A Rational Strategy for the Use of Amniotic Epithelial Stem Cell Therapy for Liver Diseases.Stem cells translational medicine · 2016Review
- The N-ethylmaleimide-sensitive factor and dysbindin interact to modulate synaptic plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Gamete derivation from embryonic stem cells, induced pluripotent stem cells or somatic cell nuclear transfer-derived embryonic stem cells: state of the art.Reproduction, fertility, and development · 2014Review
- Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties.Stem cell reviews and reports · 2014Article
- Therapeutic potential of somatic cell nuclear transfer for degenerative disease caused by mitochondrial DNA mutations.Scientific reports · 2014Article
- Article
- Tissue engineering for the oncologic urinary bladder.Nature reviews. Urology · 2012Review
- Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells.Cell reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cellular reprogramming from adult somatic cells into an embryonic cell-like state, termed induced pluripotency, has been achieved in several cell types. However, the ability to reprogram human amniotic epithelial cells (hAECs), an abundant cell source derived from discarded placental tissue, has only recently been investigated. Here we show that not only are hAECs easily reprogrammed into induced pluripotent stem cells (AE-iPSCs), but hAECs reprogram faster and more efficiently than adult and neonatal somatic dermal fibroblasts. Furthermore, AE-iPSCs express higher levels of NANOG and OCT4 compared to human foreskin fibroblast iPSCs (HFF1-iPSCs) and express decreased levels of genes associated with differentiation, including NEUROD1 and SOX17, markers of neuronal differentiation. To elucidate the mechanism behind the higher reprogramming efficiency of hAECs, we analyzed global DNA methylation, global histone acetylation, and the mitochondrial DNA A3243G point mutation. Whereas hAECs show no differences in global histone acetylation or mitochondrial point mutation accumulation compared to adult and neonatal dermal fibroblasts, hAECs demonstrate a decreased global DNA methylation compared to dermal fibroblasts. Likewise, quantitative gene expression analyses show that hAECs endogenously express OCT4, SOX2, KLF4, and c-MYC, all four factors used in cellular reprogramming. Thus, hAECs represent an ideal cell type for testing novel approaches for generating clinically viable iPSCs and offer significant advantages over postnatal cells that more likely may be contaminated by environmental exposures and infectious agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.