ArticlePloS one2012
Improvement of pest resistance in transgenic tobacco plants expressing dsRNA of an insect-associated gene EcR.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Fusion dsRNA targeting ecdysteroid signaling provides efficient and selective RNAi-based control of spider mites.Pest management science · 2026Article
- Engineering Plant-Associated Microorganisms for Bioremediation and Sustainable Agriculture.Microorganisms · 2026Review
- Disruption of yellow-e3 impairs both adult molting and cuticular melanization in the honeybee (Apis mellifera).Frontiers in zoology · 2026Article
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Sex-Biased Gene Expression of RNAi Pathway Components in the Sea Lice Caligus rogercresseyi.Marine biotechnology (New York, N.Y.) · 2025Article
- Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.Molecular biotechnology · 2024Review
- Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- RNA interference (RNAi) applications to the management of fall armyworm,Frontiers in molecular biosciences · 2022Review
- RNA Interference for Improving Disease Resistance in Plants and Its Relevance in This Clustered Regularly Interspaced Short Palindromic Repeats-Dominated Era in Terms of dsRNA-Based Biopesticides.Frontiers in plant science · 2022Review
- Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran Pests.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery.Scientific reports · 2021Article
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Double-stranded RNAs targeting inhibitor of apoptosis gene show no significant cross-species activity.Archives of insect biochemistry and physiology · 2020Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adoption of pest-resistant transgenic plants to reduce yield loss and pesticide utilization has been successful in the past three decades. Recently, transgenic plant expressing double-stranded RNA (dsRNA) targeting pest genes emerges as a promising strategy for improving pest resistance in crops. The steroid hormone, 20-hydroxyecdysone (20E), predominately controls insect molting via its nuclear receptor complex, EcR-USP. Here we report that pest resistance is improved in transgenic tobacco plants expressing dsRNA of EcR from the cotton bollworm, Helicoverpa armigera, a serious lepidopteran pest for a variety of crops. When H. armigera larvae were fed with the whole transgenic tobacco plants expressing EcR dsRNA, resistance to H. armigera was significantly improved in transgenic plants. Meanwhile, when H. armigera larvae were fed with leaves of transgenic tobacco plants expressing EcR dsRNA, its EcR mRNA level was dramatically decreased causing molting defects and larval lethality. In addition, the transgenic tobacco plants expressing H. armigera EcR dsRNA were also resistant to another lepidopteran pest, the beet armyworm, Spodoptera exigua, due to the high similarity in the nucleotide sequences of their EcR genes. This study provides additional evidence that transgenic plant expressing dsRNA targeting insect-associated genes is able to improve pest resistance.
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