ArticlePLoS genetics2012
Genome-wide functional profiling identifies genes and processes important for zinc-limited growth of Saccharomyces cerevisiae.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 70 citations in OpenAlex.
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- Understanding the action mechanisms and safety of nanoparticles with functional toxicogenomics.Nanomedicine (London, England) · 2025Article
- The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in redox regulation of intermediary metabolism, glycolysis, and zinc homeostasis.Metallomics : integrated biometal science · 2025Article
- Nutrient-dependent signaling pathways that control autophagy in yeast.FEBS letters · 2024Review
- High-throughput screening of the Saccharomyces cerevisiae genome for 2-amino-3-methylimidazo [4,5-f] quinoline resistance identifies colon cancer-associated genes.G3 (Bethesda, Md.) · 2023Article
- The NPR/Hal family of protein kinases in yeasts: biological role, phylogeny and regulation under environmental challenges.Computational and structural biotechnology journal · 2022Review
- Nutritional immunity: targeting fungal zinc homeostasis.Heliyon · 2021Review
- Yeast optimizes metal utilization based on metabolic network and enzyme kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Candida Pathogenicity and Interplay with the Immune System.Advances in experimental medicine and biology · 2021Article
- RNA-Seq Analysis of the Effect of Zinc Deficiency onFrontiers in cellular and infection microbiology · 2021Article
- Proteome reallocation from amino acid biosynthesis to ribosomes enables yeast to grow faster in rich media.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The ER-associated protease Ste24 prevents N-terminal signal peptide-independent translocation into the endoplasmic reticulum inThe Journal of biological chemistry · 2020Article
- Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inG3 (Bethesda, Md.) · 2020Article
- ZafA-mediated regulation of zinc homeostasis is required for virulence in the plant pathogen Fusarium oxysporum.Molecular plant pathology · 2020Article
- The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.Molecular microbiology · 2019Article
- The GIS2 Gene Is Repressed by a Zinc-Regulated Bicistronic RNA in Saccharomyces cerevisiae.Genes · 2018Article
- Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.PLoS genetics · 2018Article
- The Transcription Factor ZafA Regulates the Homeostatic and Adaptive Response to Zinc Starvation inGenes · 2018Article
- An Autophagy-Independent Role forGenetics · 2018Article
- Metals in fungal virulence.FEMS microbiology reviews · 2018Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Zinc is an essential nutrient because it is a required cofactor for many enzymes and transcription factors. To discover genes and processes in yeast that are required for growth when zinc is limiting, we used genome-wide functional profiling. Mixed pools of ∼4,600 deletion mutants were inoculated into zinc-replete and zinc-limiting media. These cells were grown for several generations, and the prevalence of each mutant in the pool was then determined by microarray analysis. As a result, we identified more than 400 different genes required for optimal growth under zinc-limiting conditions. Among these were several targets of the Zap1 zinc-responsive transcription factor. Their importance is consistent with their up-regulation by Zap1 in low zinc. We also identified genes that implicate Zap1-independent processes as important. These include endoplasmic reticulum function, oxidative stress resistance, vesicular trafficking, peroxisome biogenesis, and chromatin modification. Our studies also indicated the critical role of macroautophagy in low zinc growth. Finally, as a result of our analysis, we discovered a previously unknown role for the ICE2 gene in maintaining ER zinc homeostasis. Thus, functional profiling has provided many new insights into genes and processes that are needed for cells to thrive under the stress of zinc deficiency.
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Registered trials
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