ArticleJournal of bacteriology2012
Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- A genetic screen identifies a role for oprF in Pseudomonas aeruginosa biofilm stimulation by subinhibitory antibiotics.NPJ biofilms and microbiomes · 2024Article
- An Extracytoplasmic Function Sigma/Anti-Sigma Factor System Regulates β-Glucanase Expression in Tannerella forsythia in Response to Fusobacterium nucleatum Sensing.Journal of bacteriology · 2022Article
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- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits inMicrobiology spectrum · 2022Article
- Gaseous NOScientific reports · 2022Article
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- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- The Effects of Sub-inhibitory Antibiotic Concentrations onFrontiers in microbiology · 2021Article
- TacklingBiomolecules · 2020Article
- Activation of the Cell Wall Stress Response inMicroorganisms · 2020Article
- Crosstalk between the Type VI Secretion System and the Expression of Class IV Flagellar Genes in theMicroorganisms · 2020Article
- Membrane-Interactive Compounds FromFrontiers in microbiology · 2020Article
- The Temperature-Regulation ofFrontiers in microbiology · 2020Article
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Authors and funding
11 authors.
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Abstract
The OprF porin is the major outer membrane protein of Pseudomonas aeruginosa. OprF is involved in several crucial functions, including cell structure, outer membrane permeability, environmental sensing, and virulence. The oprF gene is preceded by the sigX gene, which encodes the poorly studied extracytoplasmic function (ECF) sigma factor SigX. Three oprF promoters were previously identified. Two intertwined promoters dependent on σ(70) and SigX are located in the sigX-oprF intergenic region, whereas a promoter dependent on the ECF AlgU lies within the sigX gene. An additional promoter was found in the cmpX-sigX intergenic region. In this study, we dissected the contribution of each promoter region and of each sigma factor to oprF transcription using transcriptional fusions. In Luria-Bertani (LB) medium, the oprF-proximal region (sigX-oprF intergenic region) accounted for about 80% of the oprF transcription, whereas the AlgU-dependent promoter had marginal activity. Using the sigX mutant PAOSX, we observed that the SigX-dependent promoter was largely predominant over the σ(70)-dependent promoter. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. The lack of OprF itself increased oprF transcription. Since these conditions led to cell wall alterations, oprF transcription could be activated by signals triggered by perturbation of the cell envelope.
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