ArticleApplied and environmental microbiology2012
Phenylacetic acid catabolism and its transcriptional regulation in Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Conservation, origin, and remodeling of the phenyl acetate degradation pathway.Microbial genomics · 2026Article
- Impacts of off-flavor on microbial community structure, nutritional traits and metabolite profiles in whole-plant corn silage.Frontiers in microbiology · 2026Article
- The role of the transcriptional repressor CssR inHeliyon · 2024Article
- Snowprint: a predictive tool for genetic biosensor discovery.Communications biology · 2024Article
- The TetR-type regulator AtsR is involved in multidrug response in Corynebacterium glutamicum.Microbial cell factories · 2022Article
- Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization inMicroorganisms · 2022Article
- Progress in structural and functional study of the bacterial phenylacetic acid catabolic pathway, its role in pathogenicity and antibiotic resistance.Frontiers in microbiology · 2022Review
- Transcriptional Regulation of the Creatine Utilization Genes ofFrontiers in bioengineering and biotechnology · 2022Article
- The cssR gene of Corynebacterium glutamicum plays a negative regulatory role in stress responses.Microbial cell factories · 2021Article
- Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water.Scientific reports · 2021Article
- The Operon Encoding Hydrolytic Dehalogenation of 4-Chlorobenzoate Is Transcriptionally Regulated by the TetR-Type Repressor FcbR and Its Ligand 4-Chlorobenzoyl Coenzyme A.Applied and environmental microbiology · 2021Article
- Construction of an IS-FreeFrontiers in bioengineering and biotechnology · 2021Article
- Physiological Response ofMicroorganisms · 2020Article
- On the Enigma of Glutathione-Dependent Styrene Degradation in Gordonia rubripertincta CWB2.Applied and environmental microbiology · 2018Article
- Global Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin.PloS one · 2016Article
- Involvement of the TetR-Type Regulator PaaR in the Regulation of Pristinamycin I Biosynthesis through an Effect on Precursor Supply in Streptomyces pristinaespiralis.Journal of bacteriology · 2015Article
- Involvement of the global regulator GlxR in 3-hydroxybenzoate and gentisate utilization by Corynebacterium glutamicum.Applied and environmental microbiology · 2014Article
- Role of Corynebacterium glutamicum sprA encoding a serine protease in glxR-mediated global gene regulation.PloS one · 2014Article
- RegPrecise 3.0--a resource for genome-scale exploration of transcriptional regulation in bacteria.BMC genomics · 2013Article
- Functional diversification of ROK-family transcriptional regulators of sugar catabolism in the Thermotogae phylum.Nucleic acids research · 2013Article
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are organized as a continuous paa gene cluster. The type strain of C. glutamicum, ATCC 13032, is not able to catabolize PAA, but the recombinant strain ATCC 13032/pEC-K18mob2::paa gained the ability to grow on PAA. The paaR gene, encoding a TetR family transcription regulator, was studied in detail. Disruption of paaR in strain AS 1.542 resulted in transcriptional increases of all paa genes. Transcription start sites and putative promoter regions were determined. An imperfect palindromic motif (5'-ACTNACCGNNCGNNCGGTNAGT-3'; 22 bp) was identified in the upstream regions of paa genes. Electrophoretic mobility shift assays (EMSA) demonstrated specific binding of PaaR to this motif, and phenylacetyl coenzyme A (PA-CoA) blocked binding. It was concluded that PaaR is the negative regulator of PAA degradation and that PA-CoA is the PaaR effector. In addition, GlxR binding sites were found, and binding to GlxR was confirmed. Therefore, PAA catabolism in C. glutamicum is regulated by the pathway-specific repressor PaaR, and also likely by the global transcription regulator GlxR. By comparative genomic analysis, we reconstructed orthologous PaaR regulons in 57 species, including species of Actinobacteria, Proteobacteria, and Flavobacteria, that carry PAA utilization genes and operate by conserved binding motifs, suggesting that PaaR-like regulation might commonly exist in these bacteria.
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