ArticleApplied and environmental microbiology2012
New molecular quantitative PCR assay for detection of host-specific Bifidobacteriaceae suitable for microbial source tracking.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Bacteriophages in sewage: abundance, roles, and applications.FEMS microbes · 2022Article
- Faecal pollution affects abundance and diversity of aquatic microbial community in anthropo-zoogenically influenced lotic ecosystems.Scientific reports · 2019Article
- Quantitative detection of fecal contamination with domestic poultry feces in environments in China.AMB Express · 2017Article
- Determination of crAssphage in water samples and applicability for tracking human faecal pollution.Microbial biotechnology · 2017Article
- Comparison of Microbial and Chemical Source Tracking Markers To Identify Fecal Contamination Sources in the Humber River (Toronto, Ontario, Canada) and Associated Storm Water Outfalls.Applied and environmental microbiology · 2016Article
- Evaluation of five microbial and four mitochondrial DNA markers for tracking human and pig fecal pollution in freshwater.Scientific reports · 2016Article
- Development of new host-specific Bacteroides qPCRs for the identification of fecal contamination sources in water.MicrobiologyOpen · 2016Article
- Discovering new indicators of fecal pollution.Trends in microbiology · 2014Review
- Sewage reflects the distribution of human faecal Lachnospiraceae.Environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bifidobacterium spp. belong to the commensal intestinal microbiota of warm-blooded animals. Some strains of Bifidobacterium show host specificity and have thus been proposed as host-specific targets to determine the origin of fecal pollution. Most strains have been used in microbial-source-tracking (MST) studies based on culture-dependent methods. Although some of these approaches have proved very useful, the low prevalence of culturable Bifidobacterium strains in the environment means that molecular culture-independent procedures could provide practical applications for MST. Reported here is a set of common primers and four Bifidobacterium sp. host-associated (human, cattle, pig, and poultry) probes for quantitative-PCR (qPCR) assessment of fecal source tracking. This set was tested using 25 water samples of diverse origin: urban sewage samples, wastewater from four abattoirs (porcine, bovine, and poultry), and water from a river with a low pollution load. The selected sequences showed a high degree of host specificity. There were no cross-reactions between the qPCR assays specific for each origin and samples from different fecal origins. On the basis of the findings, it was concluded that the host-specific qPCRs are sufficiently robust to be applied in environmental MST studies.
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