ArticleNucleic acids research2012
Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleases.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Nature-inspired engineering of an artificial ligase enzyme by domain fusion.Nucleic acids research · 2022Article
- Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX).Nucleic acids research · 2017Article
- Gene editing and its application for hematological diseases.International journal of hematology · 2016Review
- Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.Structure (London, England : 1993) · 2016Article
- Evaluation of TCR Gene Editing Achieved by TALENs, CRISPR/Cas9, and megaTAL Nucleases.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Massively parallel determination and modeling of endonuclease substrate specificity.Nucleic acids research · 2014Article
- Efficient design of meganucleases using a machine learning approach.BMC bioinformatics · 2014Article
- Control of catalytic efficiency by a coevolving network of catalytic and noncatalytic residues.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus.Nucleic acids research · 2014Article
- DNA cleavage enzymes for treatment of persistent viral infections: recent advances and the pathway forward.Virology · 2014Review
- Article
- megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering.Nucleic acids research · 2014Article
- Reprogramming homing endonuclease specificity through computational design and directed evolution.Nucleic acids research · 2014Article
- Engineering and flow-cytometric analysis of chimeric LAGLIDADG homing endonucleases from homologous I-OnuI-family enzymes.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Article
- Compact designer TALENs for efficient genome engineering.Nature communications · 2013Article
- High frequency targeted mutagenesis using engineered endonucleases and DNA-end processing enzymes.PloS one · 2013Article
- EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering.Nucleic acids research · 2013Article
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Authors and funding
11 authors.
Funding
Abstract
Although engineered LAGLIDADG homing endonucleases (LHEs) are finding increasing applications in biotechnology, their generation remains a challenging, industrial-scale process. As new single-chain LAGLIDADG nuclease scaffolds are identified, however, an alternative paradigm is emerging: identification of an LHE scaffold whose native cleavage site is a close match to a desired target sequence, followed by small-scale engineering to modestly refine recognition specificity. The application of this paradigm could be accelerated if methods were available for fusing N- and C-terminal domains from newly identified LHEs into chimeric enzymes with hybrid cleavage sites. Here we have analyzed the structural requirements for fusion of domains extracted from six single-chain I-OnuI family LHEs, spanning 40-70% amino acid identity. Our analyses demonstrate that both the LAGLIDADG helical interface residues and the linker peptide composition have important effects on the stability and activity of chimeric enzymes. Using a simple domain fusion method in which linker peptide residues predicted to contact their respective domains are retained, and in which limited variation is introduced into the LAGLIDADG helix and nearby interface residues, catalytically active enzymes were recoverable for ≈ 70% of domain chimeras. This method will be useful for creating large numbers of chimeric LHEs for genome engineering applications.
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