ArticleVirologica Sinica2012
Simultaneous detection of three arboviruses using a triplex RT-PCR: enzyme hybridization assay.
Article in Virologica Sinica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Co-circulation ofFrontiers in microbiology · 2023Article
- Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings.Frontiers in microbiology · 2023Article
- Establishment of a reverse transcription real-time quantitative PCR method for Getah virus detection and its application for epidemiological investigation in Shandong, China.Frontiers in microbiology · 2022Article
- A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus.Scientific reports · 2018Article
- Investigation of the genotype III to genotype I shift in Japanese encephalitis virus and the impact on human cases.Virologica Sinica · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation. We developed a cost-effective, rapid, and highly sensitive one-step "triplex RT-PCR enzyme hybridization" assay for simultaneous detections of Japanese Encephallitis virus (JEV, Flaviviridae), Getah virus (GETV, Togaviridae), and Tahyna virus (TAHV, Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction. The analytical sensitivity of this assay was 1 PFU/mL for JEV, 10 PFU/mL for GETV, and 10 PFU/mL for TAHV. This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods. When "triplex RT-PCR enzyme hybridization" was applied to 29 cerebrospinal fluid (CSF) samples that were JEV-positive by normal RT-PCR assay, all samples were strongly positive for JEV, but negative for GETV and TAHV, demonstrating a good sensitivity, specificity, and performance at CSF specimen detection.
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