ArticleThe Biochemical journal1990
Stoichiometry and stability of caldesmon in native thin filaments from sheep aorta smooth muscle.
Article in The Biochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Differential effects of thin and thick filament disruption on zebrafish smooth muscle regulatory proteins.Neurogastroenterology and motility · 2010Article
- In vitro motility of native thin filaments from Drosophila indirect flight muscles reveals that the held-up 2 TnI mutation affects calcium activation.Journal of muscle research and cell motility · 2010Article
- Identification and localization of caldesmon in cardiac muscle.The Biochemical journal · 1998Article
- Three-dimensional image reconstruction of reconstituted smooth muscle thin filaments: effects of caldesmon.Biophysical journal · 1997Article
- Modulation of gelsolin-induced actin-filament severing by caldesmon and tropomyosin and the effect of these proteins on the actin activation of myosin Mg(2+)-ATPase activity.The Biochemical journal · 1996Article
- Location of smooth-muscle myosin and tropomyosin binding sites in the C-terminal 288 residues of human caldesmon.The Biochemical journal · 1995Article
- Mode of caldesmon binding to smooth muscle thin filament: possible projection of the amino-terminal of caldesmon from native thin filament.Biophysical journal · 1995Article
- Effect of unphosphorylated smooth muscle myosin on caldesmon-mediated regulation of actin filament velocity.Journal of muscle research and cell motility · 1995Article
- Three-dimensional reconstruction of caldesmon-containing smooth muscle thin filaments.The Journal of cell biology · 1993Article
- Novel immunological technique.Journal of muscle research and cell motility · 1992Article
- Caldesmon binds to smooth muscle myosin and myosin rod and crosslinks thick filaments to actin filaments.Journal of muscle research and cell motility · 1992Article
- Functional interrelationship between calponin and caldesmon.The Biochemical journal · 1991Article
- The molecular anatomy of caldesmon.The Biochemical journal · 1991Review
- Ca(2+)-dependent regulation of vascular smooth-muscle caldesmon by S.100 and related smooth-muscle proteins.The Biochemical journal · 1991Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Ca2(+)-regulated native thin filaments were extracted from sheep aorta smooth muscle. The caldesmon content determined by quantitative gel electrophoresis was 0.06 caldesmon molecule/actin monomer (1 caldesmon molecule per 16.3 actin monomers). Dissociation of caldesmon and tropomyosin from the thin filament and the depolymerization of actin was measured by sedimenting diluted thin filaments. Actin critical concentration was 0.05 microM at 10.1 and 0.13 at 10.05 compared with 0.5 microM for pure F-actin. Tropomyosin was tightly bound, with half-maximal dissociation at less than 0.3 microM thin filaments (actin monomer) under all conditions. Caldesmon dissociation was independent of tropomyosin and not co-operative. The concentration of thin filaments where 50% of the caldesmon was dissociated (CD50) ranged from 0.2 microM (actin monomer) at 10.03 to 8 microM at 10.16 in a 5 mM-MgCl2, pH 7.1, buffer. Mg2+, 25 mM at constant I, increased CD50 4-fold. CD50 was 4-fold greater at 10(-4) M-Ca2+ than at 10(-9) M-Ca2+. Aorta heavy meromyosin (HMM).ADP.Pi complex (2.5 microM excess over thin filaments) strongly antagonized caldesmon dissociation, but skeletal-muscle HMM.ADP.Pi did not. The behaviour of caldesmon in native thin filaments was indistinguishable from caldesmon in reconstituted synthetic thin filaments. The variability of Ca2(+)-sensitivity with conditions observed in thin filament preparations was shown to be related to dissociation of regulatory caldesmon from the thin filament.
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