ArticleBMC musculoskeletal disorders2012
The effects of low dose X-irradiation on osteoblastic MC3T3-E1 cells in vitro.
Article in BMC musculoskeletal disorders, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 29 citations in OpenAlex.
- Identification of the mitochondrial protein ADCK2 as a therapeutic oncotarget of NSCLC.International journal of biological sciences · 2022Article
- Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro.Scientific reports · 2021Article
- 0.5‑Gy X‑ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts.Molecular medicine reports · 2021Article
- Low-dose X-ray irradiation induces morphological changes and cytoskeleton reorganization in osteoblasts.Experimental and therapeutic medicine · 2020Article
- Effect of autophagy on irradiation‑induced damage in osteoblast‑like MC3T3‑E1 cells.Molecular medicine reports · 2020Article
- The Influence of Radiation on Bone and Bone Cells-Differential Effects on Osteoclasts and Osteoblasts.International journal of molecular sciences · 2020Review
- Organ-Specific Effects of Low Dose Radiation Exposure: A Comprehensive Review.Frontiers in genetics · 2020Review
- Differences in responses to X-ray exposure between osteoclast and osteoblast cells.Journal of radiation research · 2017Article
- G protein-coupled receptor kinase 6 is overexpressed in glioma and promotes glioma cell proliferation.Oncotarget · 2017Article
- High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state.Lipids in health and disease · 2017Article
- The Effect of OSM on MC3T3-E1 Osteoblastic Cells in Simulated Microgravity with Radiation.PloS one · 2015Article
- Low-dose X-ray irradiation promotes osteoblast proliferation, differentiation and fracture healing.PloS one · 2014Article
- A report from Fukushima: an assessment of bone health in an area affected by the Fukushima nuclear plant incident.Journal of bone and mineral metabolism · 2013Article
- Diabetic HDL is dysfunctional in stimulating endothelial cell migration and proliferation due to down regulation of SR-BI expression.PloS one · 2012Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has been indicated that moderate or high dose of X-irradiation could delay fracture union and cause osteoradionecrosis, in part, mediated by its effect on proliferation and differentiation of osteoblasts. However, whether low dose irradiation (LDI) has similar roles on osteoblasts is still unknown. In this study, we investigated whether and to what extent LDI could affect the proliferation, differentiation and mineralization of osteoblasts in vitro.
methodsThe MC3T3-E1 cells were exposed to single dose of X-irradiation with 0, 0.1, 0.5, 1.0 Gy respectively. Cell proliferation, apoptosis, alkaline phosphatase (ALP) activity, and mineralization was evaluated by methylthiazoletetrazolium (MTT) and bromodeoxyuridine (BrdU) assay, flow cytometry, ALP viability kit and von Kossa staining, respectively. Osteocalcin (OCN) and core-binding factor α1 (Cbfα1) expressions were measured by real time-PCR and western blot, respectively.
resultsThe proliferation of the cells exposed to 2.0 Gy was significantly lower than those exposed to ≤1.0 Gy (p < 0.05) from Day 4 to Day 8, measured by MTT assay and BrdU incorporation. For cells exposed to ≤1.0 Gy, increasing dosages of X-irradiation had no significant effect on cell proliferation and apoptosis. Importantly, LDI of 0.5 and 1 Gy increased ALP activities and mineralized nodules of MC3T3-E1 cells. In addition, mRNA and protein expressions of OCN and Cbfα1 were also markedly increased after treatment with LDI at 0.5 and 1 Gy.
conclusionsLDI have different effects on proliferation and differentiation of osteoblasts from those of high dose of X-irradiation, which might suggest that LDI could lead to promotion of fracture healing through enhancing the differentiation and mineralization of osteoblasts.
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