ArticleMalaria journal2012
New Agilent platform DNA microarrays for transcriptome analysis of Plasmodium falciparum and Plasmodium berghei for the malaria research community.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Comparative clinical transcriptome of pir genes in severe Plasmodium vivax malaria.PLoS neglected tropical diseases · 2026Article
- Chromatin state dynamics during the Plasmodium falciparum intraerythrocytic development cycle.BMC genomics · 2026Article
- Cripowellins PauseMolecules (Basel, Switzerland) · 2023Article
- Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity against Plasmodium parasites.PLoS pathogens · 2022Article
- A heat-shock response regulated by the PfAP2-HS transcription factor protects human malaria parasites from febrile temperatures.Nature microbiology · 2021Article
- Multistage and transmission-blocking targeted antimalarials discovered from the open-source MMV Pandemic Response Box.Nature communications · 2021Article
- The NTP generating activity of pyruvate kinase II is critical for apicoplast maintenance ineLife · 2020Article
- A mevalonate bypass system facilitates elucidation of plastid biology in malaria parasites.PLoS pathogens · 2020Article
- Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation.BMC genomics · 2019Article
- The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression duringInternational journal of molecular sciences · 2019Article
- A seven-helix protein constitutes stress granules crucial for regulating translation during human-to-mosquito transmission of Plasmodium falciparum.PLoS pathogens · 2018Article
- Article
- Simultaneous genome-wide gene expression and transcript isoform profiling in the human malaria parasite.PloS one · 2017Article
- Transcriptional Profiling Defines Histone Acetylation as a Regulator of Gene Expression during Human-to-Mosquito Transmission of the Malaria ParasiteFrontiers in cellular and infection microbiology · 2017Article
- A cross strain Plasmodium falciparum microarray optimized for the transcriptome analysis of Plasmodium falciparum patient derived isolates.Genomics data · 2016Article
- Article
- A transcriptional switch underlies commitment to sexual development in malaria parasites.Nature · 2014Article
- Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundDNA microarrays have been a valuable tool in malaria research for over a decade but remain in limited use in part due their relatively high cost, poor availability, and technical difficulty. With the aim of alleviating some of these factors next-generation DNA microarrays for genome-wide transcriptome analysis for both Plasmodium falciparum and Plasmodium berghei using the Agilent 8 x 15 K platform were designed.
methodsProbe design was adapted from previously published methods and based on the most current transcript predictions available at the time for P. falciparum or P. berghei. Array performance and transcriptome analysis was determined using dye-coupled, aminoallyl-labelled cDNA and streamlined methods for hybridization, washing, and array analysis were developed.
resultsThe new array design marks a notable improvement in the number of transcripts covered and average number of probes per transcript. Array performance was excellent across a wide range of transcript abundance, with low inter-array and inter-probe variability for relative abundance measurements and it recapitulated previously observed transcriptional patterns. Additionally, improvements in sensitivity permitted a 20-fold reduction in necessary starting RNA amounts, further reducing experimental costs and widening the range of application.
conclusionsDNA microarrays utilizing the Agilent 8 x 15 K platform for genome-wide transcript analysis in P. falciparum and P. berghei mark an improvement in coverage and sensitivity, increased availability to the research community, and simplification of the experimental methods.
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