ArticleAMB Express2012
Branching of the p-nitrophenol (PNP) degradation pathway in burkholderia sp. Strain SJ98: Evidences from genetic characterization of PNP gene cluster.
Article in AMB Express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.Microbial cell factories · 2021Pooled it
- Characterization of the substrate specificity and regioselectivity of ring-cleavage of Pseudomonas putida DLL-E4 hydroquinone 1,2-dioxygenase (PnpC1C2).Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2025Article
- Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4.PloS one · 2022Article
- Transcriptomic analysis of Burkholderia cenocepacia CEIB S5-2 during methyl parathion degradation.Environmental science and pollution research international · 2021Article
- Physiological Role of the Previously Unexplained Benzenetriol Dioxygenase Homolog in theApplied and environmental microbiology · 2021Article
- Differential Expression and PAH Degradation: WhatInternational journal of microbiology · 2020Article
- Article
- Enhancing methyl parathion degradation by the immobilization of Burkholderia sp. isolated from agricultural soils.MicrobiologyOpen · 2017Article
- Draft Genome Sequence of Burkholderia cenocepacia Strain CEIB S5-2, a Methyl Parathion- and p-Nitrophenol-Degrading Bacterium, Isolated from Agricultural Soils in Morelos, Mexico.Genome announcements · 2016Article
- A Two-Component para-Nitrophenol Monooxygenase Initiates a Novel 2-Chloro-4-Nitrophenol Catabolism Pathway in Rhodococcus imtechensis RKJ300.Applied and environmental microbiology · 2016Article
- Biochemical Characterization of 3-Methyl-4-nitrophenol Degradation in Burkholderia sp. Strain SJ98.Frontiers in microbiology · 2016Article
- Article
- Crystal structure of PnpCD, a two-subunit hydroquinone 1,2-dioxygenase, reveals a novel structural class of Fe2+-dependent dioxygenases.The Journal of biological chemistry · 2015Article
- Draft Genome Sequence of the Organophosphorus Compound-Degrading Burkholderia zhejiangensis Strain CEIB S4-3.Genome announcements · 2014Article
- Complete Genome Sequence of the p-Nitrophenol-Degrading Bacterium Pseudomonas putida DLL-E4.Genome announcements · 2014Article
- Hydroquinone: environmental pollution, toxicity, and microbial answers.BioMed research international · 2013Review
- Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aerobic microbial degradation of p-nitrophenol (PNP) has been classically shown to proceed via 'Hydroquinone (HQ) pathway' in Gram-negative bacteria, whereas in Gram-positive PNP degraders it proceed via 'Benzenetriol (BT) pathway'. These pathways are characterized by the ring cleavage of HQ and BT as terminal aromatic intermediates respectively. Earlier reports on PNP degradation have indicated these pathways to be mutually exclusive. We report involvement of both 'HQ' and 'BT' ring cleavage pathways in PNP degradation by Burkholderia sp. strain SJ98. Genetic characterization of an ~41 Kb DNA fragment harboring PNP degradation gene cluster cloned and sequenced from strain SJ98 showed presence of multiple orfs including pnpC and pnpD which corresponded to previously characterized 'benzenetriol-dioxygenase (BtD)' and 'maleylacetate reductase (MaR)' respectively. This gene cluster also showed presence of pnpE1 and pnpE2, which shared strong sequence identity to cognate sub-units of 'hydroquinone dioxygenase' (HqD). Heterologous expression and biochemical characterization ascertained the identity of PnpE1 and PnpE2. In in vitro assay reconstituted heterotetrameric complex of PnpE1 and PnpE2 catalyzed transformation of hydroquinone (HQ) into corresponding hydroxymuconic semialdehyde (HMS) in a substrate specific manner. Together, these results clearly establish branching of PNP degradation in strain SJ98. We propose that strain SJ98 presents a useful model system for future studies on evolution of microbial degradation of PNP.
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