Evidence map›Paper›PMID 22681560›Full record

ArticleJournal of cellular and molecular medicine2012

Termination and activation of store-operated cyclic AMP production.

Isabella Maiellaro, Konstantinos Lefkimmiatis, Mary Pat Moyer, Silvana Curci, Aldebaran M Hofer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019
    Article
  7. Mitochondrial junctions with cellular organelles: CaPflugers Archiv : European journal of physiology · 2018
    Review
  8. Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  9. Review
  10. Review
  11. Article
  12. "Store-operated" cAMP signaling contributes to Ca2+-activated Cl- secretion in T84 colonic cells.American journal of physiology. Gastrointestinal and liver physiology · 2015
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Isabella MaiellaroVA Boston Healthcare System, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, West Roxbury, MA 02132, USA.
Konstantinos Lefkimmiatis
Mary Pat Moyer
Silvana Curci
Aldebaran M Hofer
Brigham and Women's Hospital · USIncell Corporation (United States) · US

Funding

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ALIMENTARY TRACTP30DK034854 · NIDDK · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI JERROLD R. TURNER · 1986 to 2026
$32.4M
Store-operated cAMP signaling in colon pathophysiologyR21DK088197 · NIDDK · HARVARD MEDICAL SCHOOL · PI HOFER, ALDEBARAN M · 2010 to 2011
$352k
Novel STIM1-dependent cyclic AMP signaling pathway in colonic epithelial functionI01BX000968 · VA · VA BOSTON HEALTH CARE SYSTEM · PI HOFER, ALDEBARAN M · 2011 to 2014
–
BLRD VA I01 BX000968NIDDK NIH HHS 1R21DK088197-01NIDDK NIH HHS 5P30DK034854-24NIDDK NIH HHS P30 DK034854NIDDK NIH HHS R21 DK088197
6 · The paper itself

Abstract

Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male fertility) have been linked to the expression of specific isoforms of the adenylyl cyclases (AC1-AC10), the enzymes that generate cyclic AMP (cAMP). Our laboratory recently discovered a new mode of cAMP production, prominent in certain cell types, that is stimulated by any manoeuvre causing reduction of free [Ca(2+) ] within the lumen of the endoplasmic reticulum (ER) calcium store. Activation of this 'store-operated' pathway requires the ER Ca(2+) sensor, STIM1, but the identity of the enzymes responsible for cAMP production and how this process is regulated is unknown. Here, we used sensitive FRET-based sensors for cAMP in single cells combined with silencing and overexpression approaches to show that store-operated cAMP production occurred preferentially via the isoform AC3 in NCM460 colonic epithelial cells. Ca(2+) entry via the plasma membrane Ca(2+) channel, Orai1, suppressed cAMP production, independent of store refilling. These findings are an important first step towards defining the functional significance and to identify the protein composition of this novel Ca(2+) /cAMP crosstalk system.

Indexed as

Adenylyl CyclasesBiosensing TechniquesCalciumCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium ChannelsCell Adhesion MoleculesCell LineCyclic AMPEndoplasmic ReticulumFluorescence Resonance Energy TransferGene Knockdown TechniquesHeLa CellsHumansMembrane ProteinsNeoplasm ProteinsORAI1 Proteinadenylate cyclase 3adenylyl cyclase 6Adenylyl Cyclasesadenylyl cyclase type VCalciumCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium ChannelsCell Adhesion MoleculesCyclic AMPMembrane ProteinsNeoplasm ProteinsORAI1 ProteinORAI1 protein, humanPertussis ToxinProtein Kinase InhibitorsSTIM1 protein, humanSTIM2 protein, humanStromal Interaction Molecule 1Stromal Interaction Molecule 2

Identifiers

PMID22681560
PMCPMC3470754
OpenAlexW2076824032

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.