ArticlePloS one2012
Transcriptome kinetics of circulating neutrophils during human experimental endotoxemia.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Identification of a forkhead box protein transcriptional network induced in human neutrophils in response to inflammatory stimuli.Frontiers in immunology · 2023Pooled it
- Aged versus fresh autologous platelet transfusion in a two-hit healthy volunteer model of transfusion-related acute lung injury.Transfusion · 2022Trial
- RNA-seq reveals activation of both common and cytokine-specific pathways following neutrophil priming.PloS one · 2013Trial
- Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Neutrophil Transcriptomic Changes in Severe Sterile Vasoplegic Syndrome Resemble a Distinct Molecular Subtype of Septic Shock.Shock (Augusta, Ga.) · 2026Article
- Expression of proBDNF/p75Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2023Article
- Neutrophils in the Pathogenesis of Rheumatoid Arthritis and Systemic Lupus Erythematosus: Same Foe Different M.O.Frontiers in immunology · 2021Review
- Immune System Disequilibrium-Neutrophils, Their Extracellular Traps, and COVID-19-Induced Sepsis.Frontiers in medicine · 2021Review
- High Purity Isolation of Low Density Neutrophils Casts Doubt on Their Exceptionality in Health and Disease.Frontiers in immunology · 2021Article
- Targeting Neutrophils in Sepsis: From Mechanism to Translation.Frontiers in pharmacology · 2021Review
- Comparison of host immune responses to LPS in human using an immune profiling panel, in vivo endotoxemia versus ex vivo stimulation.Scientific reports · 2020Article
- Neutrophils as regulators of cardiovascular inflammation.Nature reviews. Cardiology · 2020Review
- Human neutrophils depend on extrinsic factors produced by monocytes for their survival response to TLR4 stimulation.Innate immunity · 2019Article
- Pathological alteration and therapeutic implications of sepsis-induced immune cell apoptosis.Cell death & disease · 2019Review
- Immunometabolism: Another Road to Sepsis and Its Therapeutic Targeting.Inflammation · 2019Review
- Article
- Heterogeneity of neutrophils.Nature reviews. Immunology · 2019Review
- Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.PloS one · 2019Article
- Differential Gene Expression Profile of Human Neutrophils Cultured withJournal of immunology research · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymorphonuclear cells (neutrophils) play an important role in the systemic inflammatory response syndrome and the development of sepsis. These cells are essential for the defense against microorganisms, but may also cause tissue damage. Therefore, neutrophil numbers and activity are considered to be tightly regulated. Previous studies have investigated gene transcription during experimental endotoxemia in whole blood and peripheral blood mononuclear cells. However, the gene transcription response of the circulating pool of neutrophils to systemic inflammatory stimulation in vivo is currently unclear. We examined neutrophil gene transcription kinetics in healthy human subjects (n = 4) administered a single dose of endotoxin (LPS, 2 ng/kg iv). In addition, freshly isolated neutrophils were stimulated ex vivo with LPS, TNFα, G-CSF and GM-CSF to identify stimulus-specific gene transcription responses. Whole transcriptome microarray analysis of circulating neutrophils at 2, 4 and 6 hours after LPS infusion revealed activation of inflammatory networks which are involved in signaling of TNFα and IL-1α and IL-1β. The transcriptome profile of inflammatory activated neutrophils in vivo reflects extended survival and regulation of inflammatory responses. These changes in neutrophil transcriptome suggest a combination of early activation of circulating neutrophils by TNFα and G-CSF and a mobilization of young neutrophils from the bone marrow.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.