ArticlePloS one2012
Hydrogen sulfide lowers proliferation and induces protective autophagy in colon epithelial cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 105 citations in OpenAlex.
- HAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Precision nanomaterials in colorectal cancer: advancing photodynamic and photothermal therapy.RSC advances · 2025Review
- Dietary Manipulation on Gut Microbiome in Patients with Diabetes and Colorectal cancer.Current nutrition reports · 2025Review
- Investigating the antiproliferative mechanisms of NaHS, a hydrogen sulfide donor, in the SH-SY5Y cell line.Medical oncology (Northwood, London, England) · 2025Article
- Hydrogen sulfide inhibits gene expression associated with aortic valve degeneration by inducing NRF2-related pro-autophagy effect in human aortic valve interstitial cells.Molecular and cellular biochemistry · 2024Article
- A self-immolative near-infrared fluorescent probe for identification of cancer cells and facilitating its apoptosis.Analytical and bioanalytical chemistry · 2024Article
- The Triple Crown: NO, CO, and HPharmacology & therapeutics · 2023Review
- Nanomedicine-based multimodal therapies: Recent progress and perspectives in colon cancer.World journal of gastroenterology · 2023Review
- Engineering of an endogenous hydrogen sulfide responsive smart agent for photoacoustic imaging-guided combination of photothermal therapy and chemotherapy for colon cancer.Journal of advanced research · 2022Article
- Recyclable Endogenous HAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Hydrogen Sulfide and Its Donors: Keys to Unlock the Chains of Nonalcoholic Fatty Liver Disease.International journal of molecular sciences · 2022Review
- Hydrogen Sulfide Biology and Its Role in Cancer.Molecules (Basel, Switzerland) · 2022Review
- Hydrogen Sulfide: A Key Role in Autophagy Regulation from Plants to Mammalians.Antioxidants (Basel, Switzerland) · 2022Review
- Review
- The Hidden Role of Hydrogen Sulfide Metabolism in Cancer.International journal of molecular sciences · 2021Review
- Postbiotics, Metabolic Signaling, and Cancer.Molecules (Basel, Switzerland) · 2021Review
- Metabolic Influences of Gut Microbiota Dysbiosis on Inflammatory Bowel Disease.Frontiers in physiology · 2021Review
- Gut Microbiota-Derived Metabolites in Colorectal Cancer: The Bad and the Challenges.Frontiers in oncology · 2021Review
- Nutraceutical Strategies for Suppressing NLRP3 Inflammasome Activation: Pertinence to the Management of COVID-19 and Beyond.Nutrients · 2020Review
- Hydrogen Sulfide-Evoked Intracellular CaCancers · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen sulfide (H(2)S) is a gaseous bacterial metabolite that reaches high levels in the large intestine. In the present study, the effect of H(2)S on the proliferation of normal and cancerous colon epithelial cells was investigated. An immortalized colon epithelial cell line (YAMC) and a panel of colon cancer cell lines (HT-29, SW1116, HCT116) were exposed to H(2)S at concentrations similar to those found in the human colon. H(2)S inhibited normal and cancerous colon epithelial cell proliferation as measured by MTT assay. The anti-mitogenic effect of H(2)S was accompanied by G(1)-phase cell cycle arrest and the induction of the cyclin-dependent kinase inhibitor p21(Cip). Moreover, exposure to H(2)S led to features characteristic of autophagy, including increased formation of LC3B(+) autophagic vacuoles and acidic vesicular organelles as determined by immunofluorescence and acridine orange staining, respectively. Abolition of autophagy by RNA interference targeting Vps34 or Atg7 enhanced the anti-proliferative effect of H(2)S. Further mechanistic investigation revealed that H(2)S stimulated the phosphorylation of AMP-activated protein kinase (AMPK) and inhibited the phosphorylation of mammalian target of rapamycin (mTOR) and S6 kinase. Inhibition of AMPK significantly reversed H(2)S-induced autophagy and inhibition of cell proliferation. Collectively, we demonstrate that H(2)S inhibits colon epithelial cell proliferation and induces protective autophagy via the AMPK pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.