ArticlePLoS biology2012
The circadian neuropeptide PDF signals preferentially through a specific adenylate cyclase isoform AC3 in M pacemakers of Drosophila.
Article in PLoS biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
- Using UAS-Gal4 designer receptors exclusively activated by designer drugs to elucidate nondopaminergic modulation of methamphetamine-induced locomotion in Drosophila.Behavioural pharmacology · 2025Article
- Mating disrupts morning anticipation in Drosophila melanogaster females.PLoS genetics · 2022Article
- Discovery of levodopa-induced dyskinesia-associated genes using genomic studies in patients and Drosophila behavioral analyses.Communications biology · 2022Article
- Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila.PLoS genetics · 2022Article
- Regulation of Olfactory Associative Memory by the Circadian Clock Output Signal Pigment-Dispersing Factor (PDF).The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Peptidergic signaling from clock neurons regulates reproductive dormancy in Drosophila melanogaster.PLoS genetics · 2019Article
- A Symphony of Signals: Intercellular and Intracellular Signaling Mechanisms Underlying Circadian Timekeeping in Mice and Flies.International journal of molecular sciences · 2019Review
- Calcium and cAMP directly modulate the speed of the Drosophila circadian clock.PLoS genetics · 2018Article
- Membrane Currents, Gene Expression, and Circadian Clocks.Cold Spring Harbor perspectives in biology · 2017Review
- Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock.Scientific reports · 2017Article
- Enhanced sleep reverses memory deficits and underlying pathology in Drosophila models of Alzheimer's disease.Neurobiology of sleep and circadian rhythms · 2017Article
- The Narrow Abdomen Ion Channel Complex Is Highly Stable and Persists from Development into Adult Stages to Promote Behavioral Rhythmicity.Frontiers in cellular neuroscience · 2017Article
- Drosophila DH31 Neuropeptide and PDF Receptor Regulate Night-Onset Temperature Preference.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Functional PDF Signaling in the Drosophila Circadian Neural Circuit Is Gated by Ral A-Dependent Modulation.Neuron · 2016Article
- Synchrony and desynchrony in circadian clocks: impacts on learning and memory.Learning & memory (Cold Spring Harbor, N.Y.) · 2015Review
- Model Organisms in G Protein-Coupled Receptor Research.Molecular pharmacology · 2015Review
- The genetically encoded tool set for investigating cAMP: more than the sum of its parts.Frontiers in pharmacology · 2015Review
- Mmp1 processing of the PDF neuropeptide regulates circadian structural plasticity of pacemaker neurons.PLoS genetics · 2014Article
- Article
- Pigment-Dispersing Factor Signaling and Circadian Rhythms in Insect Locomotor Activity.Current opinion in insect science · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The neuropeptide Pigment Dispersing Factor (PDF) is essential for normal circadian function in Drosophila. It synchronizes the phases of M pacemakers, while in E pacemakers it decelerates their cycling and supports their amplitude. The PDF receptor (PDF-R) is present in both M and subsets of E cells. Activation of PDF-R stimulates cAMP increases in vitro and in M cells in vivo. The present study asks: What is the identity of downstream signaling components that are associated with PDF receptor in specific circadian pacemaker neurons? Using live imaging of intact fly brains and transgenic RNAi, we show that adenylate cyclase AC3 underlies PDF signaling in M cells. Genetic disruptions of AC3 specifically disrupt PDF responses: they do not affect other Gs-coupled GPCR signaling in M cells, they can be rescued, and they do not represent developmental alterations. Knockdown of the Drosophila AKAP-like scaffolding protein Nervy also reduces PDF responses. Flies with AC3 alterations show behavioral syndromes consistent with known roles of M pacemakers as mediated by PDF. Surprisingly, disruption of AC3 does not alter PDF responses in E cells--the PDF-R(+) LNd. Within M pacemakers, PDF-R couples preferentially to a single AC, but PDF-R association with a different AC(s) is needed to explain PDF signaling in the E pacemakers. Thus critical pathways of circadian synchronization are mediated by highly specific second messenger components. These findings support a hypothesis that PDF signaling components within target cells are sequestered into "circadian signalosomes," whose compositions differ between E and M pacemaker cell types.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.