ArticlePlant physiology2012
A Medicago truncatula tobacco retrotransposon insertion mutant collection with defects in nodule development and symbiotic nitrogen fixation.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 109 citations in OpenAlex.
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- A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway.The Plant cell · 2024Article
- Defense and senescence interplay in legume nodules.Plant communications · 2024Review
- Identification and Characterization of Common Bean (Plants (Basel, Switzerland) · 2023Article
- A modified aeroponic system for growing small-seeded legumes and other plants to study root systems.Plant methods · 2023Article
- The putative transporter MtUMAMIT14 participates in nodule formation in Medicago truncatula.Scientific reports · 2023Article
- Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis.Plant physiology · 2023Article
- NIN-at the heart of NItrogen-fixing Nodule symbiosis.Frontiers in plant science · 2023Review
- A collection of novelFrontiers in plant science · 2023Article
- Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Life (Basel, Switzerland) · 2022Article
- TheFrontiers in plant science · 2022Article
- NLP1 reciprocally regulates nitrate inhibition of nodulation through SUNN-CRA2 signaling inPlant communications · 2021Article
- NIN is essential for development of symbiosomes, suppression of defence and premature senescence in Medicago truncatula nodules.The New phytologist · 2021Article
- Amino Acid Polymorphisms in the VHIID Conserved Motif of Nodulation Signaling Pathways 2 Distinctly Modulate Symbiotic Signaling and Nodule Morphogenesis inFrontiers in plant science · 2021Article
- TheFrontiers in plant science · 2021Article
- A CEP Peptide Receptor-Like Kinase Regulates Auxin Biosynthesis and Ethylene Signaling to Coordinate Root Growth and Symbiotic Nodulation inThe Plant cell · 2020Article
- An integrated research framework combining genomics, systems biology, physiology, modelling and breeding for legume improvement in response to elevated COCurrent plant biology · 2020Article
- The rhizobial autotransporter determines the symbiotic nitrogen fixation activity ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Celebrating 20 Years of Genetic Discoveries in Legume Nodulation and Symbiotic Nitrogen Fixation.The Plant cell · 2020Review
- The Nodule-Specific PLAT Domain Protein NPD1 Is Required for Nitrogen-Fixing Symbiosis.Plant physiology · 2019Article
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Authors and funding
18 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Tnt1-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening of 9,300 mutant lines yielded 317 lines with putative defects in nodule development and/or nitrogen fixation. Of these, 230 lines were rescreened, and 156 lines were confirmed with defective symbiotic nitrogen fixation. Mutants were sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod-), 51 mutants with totally ineffective nodules (Nod+ Fix-), 17 mutants with partially ineffective nodules (Nod+ Fix+/-), 27 mutants defective in nodule emergence, elongation, and nitrogen fixation (Nod+/- Fix-), one mutant with delayed and reduced nodulation but effective in nitrogen fixation (dNod+/- Fix+), and 11 supernodulating mutants (Nod++Fix+/-). A total of 2,801 flanking sequence tags were generated from the 156 symbiotic mutant lines. Analysis of flanking sequence tags revealed 14 insertion alleles of the following known symbiotic genes: NODULE INCEPTION (NIN), DOESN'T MAKE INFECTIONS3 (DMI3/CCaMK), ERF REQUIRED FOR NODULATION, and SUPERNUMERARY NODULES (SUNN). In parallel, a polymerase chain reaction-based strategy was used to identify Tnt1 insertions in known symbiotic genes, which revealed 25 additional insertion alleles in the following genes: DMI1, DMI2, DMI3, NIN, NODULATION SIGNALING PATHWAY1 (NSP1), NSP2, SUNN, and SICKLE. Thirty-nine Nod- lines were also screened for arbuscular mycorrhizal symbiosis phenotypes, and 30 mutants exhibited defects in arbuscular mycorrhizal symbiosis. Morphological and developmental features of several new symbiotic mutants are reported. The collection of mutants described here is a source of novel alleles of known symbiotic genes and a resource for cloning novel symbiotic genes via Tnt1 tagging.
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