ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2012
Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Trial
- Aetiology of acute respiratory infection in Vientiane, Lao PDR, from a case-control study.Scientific reports · 2026Article
- Avian Metapneumovirus: Virology, Epidemiology, and Insights from a Comparative Analysis with Human Metapneumovirus-A Review.Biomolecules · 2026Review
- Article
- Review: Knowledge Gained and Gaps in Understanding in the 25 Years Since Human Metapneumovirus Was First Identified as a Cause of Human Disease.The Journal of infectious diseases · 2025Review
- Molecular epidemiology, coinfection, and diversity of enteroviruses detected in respiratory samples collected from patients with influenza-like illness in Hangzhou, China, in 2023.Virology journal · 2025Article
- Severe Human Herpesvirus 7 Infection in Healthy Children.Fukushima journal of medical science · 2025Article
- Seasonality and Genotype Diversity of Human Rhinoviruses during an Eight-Year Period in Slovenia.Microorganisms · 2024Article
- Coinfection of SARS-CoV-2 with other respiratory pathogens in outpatients from Ecuador.Frontiers in public health · 2023Article
- Impact of a Rapid Respiratory Pathogen Panel on Antibiotic and Chest Radiography Usage and Hospital Length of Stay in the Pediatric Inpatient Setting.The Permanente journal · 2022Article
- Clinical manifestations and outcome of viral acute lower respiratory infection in hospitalised children in Myanmar.BMC infectious diseases · 2022Article
- Assessing the Impact of Acute Respiratory Illnesses on the Risk of Subsequent Respiratory Illness.The Journal of infectious diseases · 2022Article
- Viral Coinfection and Nasal Cytokines in Children With Clinically Diagnosed Acute Sinusitis.Frontiers in pediatrics · 2021Article
- Human Respiratory Syncytial Virus Detected in Mountain Gorilla Respiratory Outbreaks.EcoHealth · 2020Article
- Respiratory Syncytial Virus-Associated Hospitalizations Among Young Children: 2015-2016.Pediatrics · 2020Article
- The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults.Virology journal · 2018Article
- Clinical Features of Human Metapneumovirus Infection in Ambulatory Children Aged 5-13 Years.Journal of the Pediatric Infectious Diseases Society · 2018Article
- Human metapneumovirus - what we know now.F1000Research · 2018Review
- Chest radiographic features of human metapneumovirus infection in pediatric patients.Pediatric radiology · 2017Article
- Nasopharyngeal Pneumococcal Density Is Associated With Viral Activity but Not With Use of Improved Stoves Among Young Andean Children.Open forum infectious diseases · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHuman metapneumovirus (HMPV) is a paramyxovirus with multiple genetic lineages that is a leading cause of acute respiratory disease. Several RT-PCR assays have been described based on limited available sequence data.
objectivesTo develop a broadly reactive real-time RT-PCR assay for HMPV that allows for a rapid, sensitive, and specific detection in a clinical or research setting. STUDY
designThree published assays for HMPV were modified based on analysis of multiple HMPV sequences obtained from GenBank. Original and modified assays were tested against prototype HMPV strains from each genetic sublineage, multiple isolates of HMPV from different years, a collection of clinical specimens, and commercial validation panels.
resultsA number of potential sequence mismatches with diverse HMPV strains were identified. Modifications were made to oligonucleotides to improve annealing efficiency. Primers and probes based on newer sequence data offered enhanced detection of all subgroups, especially for low titer specimens. The new primers and probe detected multiple clinical isolates of HMPV collected over a twenty-year period. The modified assay improved detection of HMPV in a panel of clinical specimens, and correctly identified HMPV samples in two commercial validation sets.
conclusionsWe report a modified real-time RT-PCR assay for HMPV that detects all genetic lineages with high sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.