ArticleJournal of translational medicine2012
Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery.
Article in Journal of translational medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 160 citations in OpenAlex.
- Targeted nanotherapeutic strategy for melanoma using silk-fibroin based artemisinin nanoparticles.RSC advances · 2026Article
- Dynamic Silk Fibroin Hydrogels for Programmable Bioactuation and Smart Shape Deformation: Mechanisms, Performance Evaluation, and Biomedical Applications.Gels (Basel, Switzerland) · 2026Review
- Characterization and Migration Activity of Thermoresponsive Silk Fibroin-Aloe Vera Gel in Normal and Diabetic Fibroblasts.Gels (Basel, Switzerland) · 2026Article
- A cell-laden methacrylated chitosan-based photocrosslinkable hydrogel for bone tissue engineering and its in vitro structural and biological characterization.Scientific reports · 2026Article
- Functionalized Silk Fibroin and Mucin Hybrid Material for Targeted EGF and Papain Delivery in Wound Healing.ACS omega · 2025Article
- Silk Fibroin-Alginate Aerogel Beads Produced by Supercritical COGels (Basel, Switzerland) · 2025Article
- Development of a Sensory Neuron-Integrated Skin Spheroid Model for the Evaluation of Neuropeptide-Based Topical Delivery Systems.ACS biomaterials science & engineering · 2025Article
- Preparation of antimicrobial polymeric composites using defective silk cocoons and moringa seed oil as additives for polyvinyl chloride.Scientific reports · 2025Article
- Article
- Unveiling the multifaceted potential of amyloid fibrils: from pathogenic myths to biotechnological marvels.Biophysical reviews · 2024Review
- A Novel Triad of Bio-Inspired Design, Digital Fabrication, and Bio-Derived Materials for Personalised Bone Repair.Materials (Basel, Switzerland) · 2024Review
- Effect of Silk Fibroin on the Mechanical and Transport Properties of Agarose Hydrogels.Gels (Basel, Switzerland) · 2024Article
- Article
- Precisely Printable Silk Fibroin/Carboxymethyl Cellulose/Alginate Bioink for 3D Printing.Polymers · 2024Article
- Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting.Materials today. Bio · 2024Article
- Psidium guajava L. phenolic compound-reinforced lamellar scaffold for tracheal tissue engineering.Drug delivery and translational research · 2024Article
- Silk Fibroin/ZnO Coated TiOMaterials (Basel, Switzerland) · 2023Article
- Advances in Preparation and Properties of Regenerated Silk Fibroin.International journal of molecular sciences · 2023Review
- Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases.Polymers · 2023Article
- Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents.ACS omega · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDegummed silk fibroin from Bombyx mori (silkworm) has potential carrier capabilities for drug delivery in humans; however, the processing methods have yet to be comparatively analyzed to determine the differential effects on the silk protein properties, including crystalline structure and activity.
methodsIn this study, we treated degummed silk with four kinds of calcium-alcohol solutions, and performed secondary structure measurements and enzyme activity test to distinguish the differences between the regenerated fibroins and degummed silk fibroin.
resultsGel electrophoresis analysis revealed that Ca(NO3)2-methanol, Ca(NO3)2-ethanol, or CaCl2-methanol treatments produced more lower molecular weights of silk fibroin than CaCl2-ethanol. X-ray diffraction and Fourier-transform infrared spectroscopy showed that CaCl2-ethanol produced a crystalline structure with more silk I (α-form, type II β-turn), while the other treatments produced more silk II (β-form, anti-parallel β-pleated sheet). Solid-State 13C cross polarization and magic angle spinning-nuclear magnetic resonance measurements suggested that regenerated fibroins from CaCl2-ethanol were nearly identical to degummed silk fibroin, while the other treatments produced fibroins with significantly different chemical shifts. Finally, enzyme activity test indicated that silk fibroins from CaCl2-ethanol had higher activity when linked to a known chemotherapeutic drug, L-asparaginase, than the fibroins from other treatments.
conclusionsCollectively, these results suggest that the CaCl2-ethanol processing method produces silk fibroin with biomaterial properties that are appropriate for drug delivery.
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Registered trials
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