ArticleVeterinary research2012
Improved strategy for phylogenetic analysis of classical swine fever virus based on full-length E2 encoding sequences.
Article in Veterinary research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- Efficacy Evaluation of an E2 Subunit Vaccine Against Highly Virulent Classical Swine Fever Virus Strain.Vaccines · 2025Article
- Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform.Pathogens (Basel, Switzerland) · 2025Article
- PorvacArchives of virology · 2024Article
- Rapid Differential Detection of Wild-Type Classical Swine Fever Virus and Hog Cholera Lapinized Virus Vaccines by TaqMan MGB-Based Dual One-Step Real-Time RT-PCR.Veterinary sciences · 2024Article
- Article
- Cross-reactivities and cross-neutralization of different envelope glycoproteins E2 antibodies against different genotypes of classical swine fever virus.Frontiers in veterinary science · 2023Article
- The Diversity and Spatiotemporally Evolutionary Dynamic of Atypical Porcine Pestivirus in China.Frontiers in microbiology · 2022Article
- Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever virus.Frontiers in immunology · 2022Article
- Molecular Epidemiology Questions Transmission Pathways Identified During the Year 2000 Outbreak of Classical Swine Fever in the UK.Frontiers in microbiology · 2022Article
- Complete genome sequences of classical swine fever virus: Phylogenetic and evolutionary analyses.Frontiers in microbiology · 2022Article
- Phylogenetic analysis of classical swine fever virus isolates from China.Archives of virology · 2021Article
- Review
- Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016-2018.Viruses · 2021Article
- Pathology of the outbreak of subgenotype 2.5 classical swine fever virus in northern Vietnam.Veterinary medicine and science · 2021Article
- Analysis of Virus Population Profiles within Pigs Infected with Virulent Classical Swine Fever Viruses: Evidence for Bottlenecks in Transmission but Absence of Tissue-Specific Virus Variants.Journal of virology · 2020Article
- Genomic characteristics of classical swine fever virus strains of bovine origin according to primary and secondary sequence-structure analysis.Open veterinary journal · 2020Article
- Investigation of chronic and persistent classical swine fever infections under field conditions and their impact on vaccine efficacy.BMC veterinary research · 2019Article
- Reemergence of Classical Swine Fever, Japan, 2018.Emerging infectious diseases · 2019Article
- Bungowannah virus in the affected pig population: a retrospective genetic analysis.Virus genes · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular epidemiology has proven to be an essential tool in the control of classical swine fever (CSF) and its use has significantly increased during the past two decades. Phylogenetic analysis is a prerequisite for virus tracing and thus allows implementing more effective control measures. So far, fragments of the 5´NTR (150 nucleotides, nt) and the E2 gene (190 nt) have frequently been used for phylogenetic analyses. The short sequence lengths represent a limiting factor for differentiation of closely related isolates and also for confidence levels of proposed CSFV groups and subgroups. In this study, we used a set of 33 CSFV isolates in order to determine the nucleotide sequences of a 3508-3510 nt region within the 5´ terminal third of the viral genome. Including 22 additional sequences from GenBank database different regions of the genome, comprising the formerly used short 5´NTR and E2 fragments as well as the genomic regions encoding the individual viral proteins Npro, C, Erns, E1, and E2, were compared with respect to variability and suitability for phylogenetic analysis. Full-length E2 encoding sequences (1119 nt) proved to be most suitable for reliable and statistically significant phylogeny and analyses revealed results as good as obtained with the much longer entire 5´NTR-E2 sequences. This strategy is therefore recommended by the EU and OIE Reference Laboratory for CSF as it provides a solid and improved basis for CSFV molecular epidemiology. Finally, the power of this method is illustrated by the phylogenetic analysis of closely related CSFV isolates from a recent outbreak in Lithuania.
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