ArticleFEMS microbiology letters2012
Genetic transformation of Neisseria gonorrhoeae shows a strand preference.
Article in FEMS microbiology letters, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2022Article
- Article
- Single-Stranded DNA Uptake during Gonococcal Transformation.Journal of bacteriology · 2016Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis.Molecular cell · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Natural transformation is the main means of horizontal genetic exchange in the obligate human pathogen Neisseria gonorrhoeae. Neisseria spp. have been shown to preferentially take up and transform their own DNA by recognizing a non-palindromic 10 or 12 nucleotide DNA uptake sequence (DUS10 or DUS12). We investigated the ability of the DUS12 to enhance single-stranded DNA (ssDNA) transformation. Given the non-palindromic nature of the DUS12, we tested whether both strands of the DUS equally enhance transformation. Recombinant single-stranded M13 phage harboring transforming DNA with the Watson DUS12, the Crick DUS12, or no DUS (DUS0) were constructed and circular ssDNA was purified. Southern blots of the purified DNA probed with strand-specific oligonucleotide probes showed > 10,000 : 1 ratio of ssDNA to contaminating dsDNA. The Crick strand of the DUS12 enhanced ssDNA transformation 180- to 470-fold over DUS0 ssDNA, whereas the Watson strand of the DUS only modestly enhanced ssDNA transformation in two strains of N. gonorrhoeae. These data confirm that ssDNA efficiently transforms N. gonorrhoeae, but that there is a strand preference and that part of this strand preference is a greater efficiency of the Crick strand of the DUS12 in enhancing transformation.
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Registered trials
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