ArticleJournal of the American Chemical Society2012
Pulsed ESR dipolar spectroscopy for distance measurements in immobilized spin labeled proteins in liquid solution.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
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- A dynamic duo: Copper metalloregulators and continuous-wave electron spin resonance spectroscopy.Biophysical reports · 2026Review
- Detection of Mutation-Induced Conformational Changes in an Intrinsically Disordered Protein by Double Quantum Coherence Electron Spin Resonance Methodology.Journal of the American Chemical Society · 2026Article
- Effect of Hydrostatic Pressure on theThe journal of physical chemistry letters · 2025Article
- Celebration of 80 years of EPR.Applied magnetic resonance · 2024Article
- An analysis of double-quantum coherence ESR in an N-spin system: Analytical expressions and predictions.The Journal of chemical physics · 2024Article
- Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy Distance Measurements at Low Nanomolar Concentrations: The CuThe journal of physical chemistry letters · 2024Article
- Cucurbit[7]uril Enhances Distance Measurements of Spin-Labeled Proteins.Journal of the American Chemical Society · 2023Article
- A newJournal of magnetic resonance (San Diego, Calif. : 1997) · 2023Article
- GdJournal of the American Chemical Society · 2023Article
- Intermolecular contributions, filtration effects and signal composition of SIFTER (single-frequency technique for refocusing).Magnetic resonance (Gottingen, Germany) · 2023Article
- Synthesis and characterization of a biocompatibleThe Analyst · 2022Article
- Pulse dipolar EPR for determining nanomolar binding affinities.Chemical communications (Cambridge, England) · 2022Article
- Synthesis and characterization of a Cu(ii) coordination-containing TAM radical as a nitroxyl probe.RSC advances · 2022Article
- Impact of Chlorine Substitution on Electron Spin Relaxation of a Trityl Radical.Applied magnetic resonance · 2022Article
- Is Cys(MTSL) the Best α-Amino Acid Residue to Electron Spin Labeling of Synthetically Accessible Peptide Molecules with Nitroxides?ACS omega · 2022Article
- Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins: CuThe journal of physical chemistry. B · 2021Article
- Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance MeasurementsThe journal of physical chemistry. B · 2021Article
8 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Pulsed electron spin resonance (ESR) dipolar spectroscopy (PDS) in combination with site-directed spin labeling is unique in providing nanometer-range distances and distributions in biological systems. To date, most of the pulsed ESR techniques require frozen solutions at cryogenic temperatures to reduce the rapid electron spin relaxation rate and to prevent averaging of electron-electron dipolar interaction due to the rapid molecular tumbling. To enable measurements in liquid solution, we are exploring a triarylmethyl (TAM)-based spin label with a relatively long relaxation time where the protein is immobilized by attachment to a solid support. In this preliminary study, TAM radicals were attached via disulfide linkages to substituted cysteine residues at positions 65 and 80 or 65 and 76 in T4 lysozyme immobilized on Sepharose. Interspin distances determined using double quantum coherence (DQC) in solution are close to those expected from models, and the narrow distance distribution in each case indicates that the TAM-based spin label is relatively localized.
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