ArticleInternational journal of evolutionary biology2012
Whole-Genome Profiling of a Novel Mutagenesis Technique Using Proofreading-Deficient DNA Polymerase δ.
Article in International journal of evolutionary biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Epistatic impacts of cis- and trans-regulatory mutations on the distribution of mutational effects for gene expression in Saccharomyces cerevisiae.Molecular biology and evolution · 2026Article
- Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology.Archives of microbiology · 2026Review
- Construction of yeast with extremely high 2,3-butanediol tolerance by introducing point and structural mutations and partial elucidation of the mechanism of 2,3-butanediol tolerance.Applied microbiology and biotechnology · 2025Article
- Article
- Biosensor-CoupledmSystems · 2022Article
- Mutational sources ofeLife · 2021Article
- Whole-genome sequencing of Puccinia striiformis f. sp. tritici mutant isolates identifies avirulence gene candidates.BMC genomics · 2020Article
- Generation ofJournal of applied glycoscience · 2019Article
- Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application.Journal of industrial microbiology & biotechnology · 2018Article
- Genomic saturation mutagenesis and polygenic analysis identify novel yeast genes affecting ethyl acetate production, a non-selectable polygenic trait.Microbial cell (Graz, Austria) · 2016Article
- Analysis of spontaneous suppressor mutants from the photomixotrophically grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp. PCC 6803.Photosynthesis research · 2015Article
- Scanning the effects of ethyl methanesulfonate on the whole genome of Lotus japonicus using second-generation sequencing analysis.G3 (Bethesda, Md.) · 2015Article
- Confirmation of the cellular targets of benomyl and rapamycin using next-generation sequencing of resistant mutants in S. cerevisiae.Molecular bioSystems · 2014Article
- Generation of rodent malaria parasites with a high mutation rate by destructing proofreading activity of DNA polymerase δ.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Whole genome profiling of spontaneous and chemically induced mutations in Toxoplasma gondii.BMC genomics · 2014Article
- Implications of fidelity difference between the leading and the lagging strand of DNA for the acceleration of evolution.Frontiers in oncology · 2012Article
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5 authors.
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Abstract
A novel mutagenesis technique using error-prone DNA polymerase δ (polδ), the disparity mutagenesis model of evolution, has been successfully employed to generate novel microorganism strains with desired traits. However, little else is known about the spectra of mutagenic effects caused by disparity mutagenesis. We evaluated and compared the performance of the polδMKII mutator, which expresses the proofreading-deficient and low-fidelity polδ, in Saccharomyces cerevisiae haploid strain with that of the commonly used chemical mutagen ethyl methanesulfonate (EMS). This mutator strain possesses exogenous mutant polδ supplied from a plasmid, tthereby leaving the genomic one intact. We measured the mutation rate achieved by each mutagen and performed high-throughput next generation sequencing to analyze the genome-wide mutation spectra produced by the 2 mutagenesis methods. The mutation frequency of the mutator was approximately 7 times higher than that of EMS. Our analysis confirmed the strong G/C to A/T transition bias of EMS, whereas we found that the mutator mainly produces transversions, giving rise to more diverse amino acid substitution patterns. Our present study demonstrated that the polδMKII mutator is a useful and efficient method for rapid strain improvement based on in vivo mutagenesis.
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