ArticlePloS one2012
Apelin enhances directed cardiac differentiation of mouse and human embryonic stem cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- International Union of Basic and Clinical Pharmacology. CXXI. Apelin receptor pharmacology in the human cardiovascular system and emerging clinical applications.Pharmacological reviews · 2026Review
- Assessment of the feasibility of human amniotic membrane stem cell-derived cardiomyocytes in vitro.Heliyon · 2024Article
- Inducible apelin receptor knockdown reduces differentiation efficiency and contractility of hESC-derived cardiomyocytes.Cardiovascular research · 2023Article
- Apelin-13 regulates angiotensin ii-induced Cx43 downregulation and autophagy via the AMPK/mTOR signaling pathway in HL-1 cells.Physiological research · 2020Article
- Apela improves cardiac and renal function in mice with acute myocardial infarction.Journal of cellular and molecular medicine · 2020Article
- Elimination of human folypolyglutamate synthetase alters programming and plasticity of somatic cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Electrical Stimulation Enhances Cardiac Differentiation of Human Induced Pluripotent Stem Cells for Myocardial Infarction Therapy.Antioxidants & redox signaling · 2018Article
- Apelin Ameliorates High Glucose-Induced Downregulation of Connexin 43 via AMPK-Dependent Pathway in Neonatal Rat Cardiomyocytes.Aging and disease · 2018Article
- Hypoxia preconditioning promotes cardiac stem cell survival and cardiogenic differentiation in vitro involving activation of the HIF-1α/apelin/APJ axis.Stem cell research & therapy · 2017Article
- Apelin-13 infusion salvages the peri-infarct region to preserve cardiac function after severe myocardial injury.International journal of cardiology · 2016Article
- Cardiotrophin-1 promotes cardiomyocyte differentiation from mouse induced pluripotent stem cells via JAK2/STAT3/Pim-1 signaling pathway.Journal of geriatric cardiology : JGC · 2015Article
- Serum apelin is associated with left ventricular hypertrophy in untreated hypertension patients.Journal of translational medicine · 2015Article
- Effects of apelin on the cardiovascular system.Heart failure reviews · 2015Review
- Direct evaluation of myocardial viability and stem cell engraftment demonstrates salvage of the injured myocardium.Circulation research · 2015Article
- G-protein Coupled Receptor Signaling in Pluripotent Stem Cell-derived Cardiovascular Cells: Implications for Disease Modeling.Frontiers in cell and developmental biology · 2015Review
- Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.PloS one · 2015Article
- Tri-iodo-l-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells.Journal of molecular and cellular cardiology · 2014Article
- Pluripotent stem cell derived cardiomyocytes for cardiac repair.Current treatment options in cardiovascular medicine · 2014Article
- Measuring the contractile forces of human induced pluripotent stem cell-derived cardiomyocytes with arrays of microposts.Journal of biomechanical engineering · 2014Article
- Toddler: an embryonic signal that promotes cell movement via Apelin receptors.Science (New York, N.Y.) · 2014Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Apelin is a peptide ligand for an orphan G-protein coupled receptor (APJ receptor) and serves as a critical gradient for migration of mesodermal cells fated to contribute to the myocardial lineage. The present study was designed to establish a robust cardiac differentiation protocol, specifically, to evaluate the effect of apelin on directed differentiation of mouse and human embryonic stem cells (mESCs and hESCs) into cardiac lineage. Different concentrations of apelin (50, 100, 500 nM) were evaluated to determine its differentiation potential. The optimized dose of apelin was then combined with mesodermal differentiation factors, including BMP-4, activin-A, and bFGF, in a developmentally specific temporal sequence to examine the synergistic effects on cardiac differentiation. Cellular, molecular, and physiologic characteristics of the apelin-induced contractile embryoid bodies (EBs) were analyzed. It was found that 100 nM apelin resulted in highest percentage of contractile EB for mESCs while 500 nM had the highest effects on hESCs. Functionally, the contractile frequency of mESCs-derived EBs (mEBs) responded appropriately to increasing concentration of isoprenaline and diltiazem. Positive phenotype of cardiac specific markers was confirmed in the apelin-treated groups. The protocol, consisting of apelin and mesodermal differentiation factors, induced contractility in significantly higher percentage of hESC-derived EBs (hEBs), up-regulated cardiac-specific genes and cell surface markers, and increased the contractile force. In conclusion, we have demonstrated that the treatment of apelin enhanced cardiac differentiation of mouse and human ESCs and exhibited synergistic effects with mesodermal differentiation factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.