ArticlePloS one2012
Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle.Science advances · 2025Article
- Mechanisms of Synaptic Vesicle Exo- and Endocytosis.Biomedicines · 2022Review
- FMRP Sustains Presynaptic Function via Control of Activity-Dependent Bulk Endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Inositol hexakisphosphate kinases differentially regulate trafficking of vesicular glutamate transporters 1 and 2.Frontiers in cellular neuroscience · 2022Article
- Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses.Nature communications · 2021Article
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- Activity-dependent bulk endocytosis proteome reveals a key presynaptic role for the monomeric GTPase Rab11.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Synaptic Vesicle Endocytosis in Different Model Systems.Frontiers in cellular neuroscience · 2018Review
- A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis.PLoS biology · 2017Article
- VGLUT2 Trafficking Is Differentially Regulated by Adaptor Proteins AP-1 and AP-3.Frontiers in cellular neuroscience · 2017Article
- Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB.Nature communications · 2016Article
- Endosome-mediated endocytic mechanism replenishes the majority of synaptic vesicles at mature CNS synapses in an activity-dependent manner.Scientific reports · 2016Article
- Monitoring activity-dependent bulk endocytosis with the genetically-encoded reporter VAMP4-pHluorin.Journal of neuroscience methods · 2016Article
- Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis.Journal of neurochemistry · 2015Article
- Advances in chemical labeling of proteins in living cells.Cell and tissue research · 2015Review
- Calcineurin regulates the yeast synaptojanin Inp53/Sjl3 during membrane stress.Molecular biology of the cell · 2015Article
- Acute increase of α-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation.Molecular biology of the cell · 2014Article
- Vesicles derived via AP-3-dependent recycling contribute to asynchronous release and influence information transfer.Nature communications · 2014Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
To maintain neurotransmission in central neurons, several mechanisms are employed to retrieve synaptically exocytosed membrane. The two major modes of synaptic vesicle (SV) retrieval are clathrin-mediated endocytosis and activity-dependent bulk endocytosis (ADBE). ADBE is the dominant SV retrieval mode during intense stimulation, however the precise physiological conditions that trigger this mode are not resolved. To determine these parameters we manipulated rat hippocampal neurons using a wide spectrum of stimuli by varying both the pattern and duration of stimulation. Using live-cell fluorescence imaging and electron microscopy approaches, we established that stimulation frequency, rather than the stimulation load, was critical in the triggering of ADBE. Thus two hundred action potentials, when delivered at high frequency, were sufficient to induce near maximal bulk formation. Furthermore we observed a strong correlation between SV pool size and ability to perform ADBE. We also identified that inhibitory nerve terminals were more likely to utilize ADBE and had a larger SV recycling pool. Thus ADBE in hippocampal synaptic terminals is tightly coupled to stimulation frequency and is more likely to occur in terminals with large SV pools. These results implicate ADBE as a key modulator of both hippocampal neurotransmission and plasticity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.