ArticlePloS one2012
Cycling of Etk and Etp phosphorylation states is involved in formation of group 4 capsule by Escherichia coli.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei.Nature communications · 2025Article
- Molecular basis for the phosphorylation of bacterial tyrosine kinase Wzc.Nature communications · 2025Article
- Tyrosine Kinases and Phosphatases: Enablers of theFrontiers in oral health · 2022Review
- Surviving Serum: the Escherichia coliInfection and immunity · 2021Article
- The molecular basis of regulation of bacterial capsule assembly by Wzc.Nature communications · 2021Article
- Article
- Porphyromonas gingivalis Tyrosine Phosphatase Php1 Promotes Community Development and Pathogenicity.mBio · 2019Article
- Global landscape of cell envelope protein complexes in Escherichia coli.Nature biotechnology · 2018Article
- Structure-function aspects of the Porphyromonas gingivalis tyrosine kinase Ptk1.Molecular oral microbiology · 2017Article
- Tyrosine Phosphorylation and Dephosphorylation in Burkholderia cenocepacia Affect Biofilm Formation, Growth under Nutritional Deprivation, and Pathogenicity.Applied and environmental microbiology · 2016Article
- Characterization of a bacterial tyrosine kinase in Porphyromonas gingivalis involved in polymicrobial synergy.MicrobiologyOpen · 2014Article
- The role of bacterial protein tyrosine phosphatases in the regulation of the biosynthesis of secreted polysaccharides.Antioxidants & redox signaling · 2014Review
- Microbial protein-tyrosine kinases.The Journal of biological chemistry · 2014Review
- Escherichia coli deletion mutants illuminate trade-offs between growth rate and flux through a foreign anabolic pathway.PloS one · 2014Article
- Regulatory interactions between a bacterial tyrosine kinase and its cognate phosphatase.The Journal of biological chemistry · 2013Article
- Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli.Structure (London, England : 1993) · 2013Article
- Wzb of Vibrio vulnificus represents a new group of low-molecular-weight protein tyrosine phosphatases with a unique insertion in the W-loop.The Journal of biological chemistryArticle
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Authors and funding
9 authors.
Funding
Abstract
Capsules frequently play a key role in bacterial interactions with their environment. Escherichia coli capsules were categorized as groups 1 through 4, each produced by a distinct mechanism. Etk and Etp are members of protein families required for the production of group 1 and group 4 capsules. These members function as a protein tyrosine kinase and protein tyrosine phosphatase, respectively. We show that Etp dephosphorylates Etk in vivo, and mutations rendering Etk or Etp catalytically inactive result in loss of group 4 capsule production, supporting the notion that cyclic phosphorylation and dephosphorylation of Etk is required for capsule formation. Notably, Etp also becomes tyrosine phosphorylated in vivo and catalyzes rapid auto-dephosphorylation. Further analysis identified Tyr121 as the phosphorylated residue of Etp. Etp containing Phe, Glu or Ala in place of Tyr121 retained phosphatase activity and catalyzed dephosphorylation of Etp and Etk. Although EtpY121E and EtpY121A still supported capsule formation, EtpY121F failed to do so. These results suggest that cycles of phosphorylation and dephosphorylation of Etp, as well as Etk, are involved in the formation of group 4 capsule, providing an additional regulatory layer to the complex control of capsule production.
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