ArticleJournal of the Royal Society, Interface2012
Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage.
Article in Journal of the Royal Society, Interface, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- Role of collagen VI and IX in mechanotransduction in the cartilage microenvironments: A multiscale in silico examination.Journal of orthopaedic translation · 2026Article
- A damaging mutation in COL6A3 alters the mechanobiologic response of chondrocytes derived from human induced pluripotent stem cells.Stem cell research & therapy · 2026Article
- Ultrafast crosslinking, strongly adhesiveBioactive materials · 2026Article
- Structure, Mechanics, and Mechanobiology of Fibrocartilage Pericellular Matrix Mediated by Type V Collagen.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage.ACS biomaterials science & engineering · 2024Article
- Rapid specialization and stiffening of the primitive matrix in developing articular cartilage and meniscus.Acta biomaterialia · 2023Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Impact of perlecan, a core component of basement membrane, on regeneration of cartilaginous tissues.Acta biomaterialia · 2021Review
- Perlecan in Pericellular Mechanosensory Cell-Matrix Communication, Extracellular Matrix Stabilisation and Mechanoregulation of Load-Bearing Connective Tissues.International journal of molecular sciences · 2021Review
- Decorin regulates cartilage pericellular matrix micromechanobiology.Matrix biology : journal of the International Society for Matrix Biology · 2021Article
- Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte-Hydrogel Interface.Advanced functional materials · 2020Article
- Nanomechanical Properties of Articular Cartilage Due to the PRP Injection in Experimental Osteoarthritis in Rabbits.Molecules (Basel, Switzerland) · 2020Article
- Early changes in cartilage pericellular matrix micromechanobiology portend the onset of post-traumatic osteoarthritis.Acta biomaterialia · 2020Article
- AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness.Materials (Basel, Switzerland) · 2020Article
- Type III collagen is a key regulator of the collagen fibrillar structure and biomechanics of articular cartilage and meniscus.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Effects of dietary fatty acid content on humeral cartilage and bone structure in a mouse model of diet-induced obesity.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Osteoarthritis as a disease of the cartilage pericellular matrix.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Microscale mapping of extracellular matrix elasticity of mouse joint cartilage: an approach to extracting bulk elasticity of soft matter with surface roughness.Soft matter · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The pericellular matrix (PCM) is a narrow region that is rich in type VI collagen that surrounds each chondrocyte within the extracellular matrix (ECM) of articular cartilage. Previous studies have demonstrated that the chondrocyte micromechanical environment depends on the relative properties of the chondrocyte, its PCM and the ECM. The objective of this study was to measure the influence of type VI collagen on site-specific micromechanical properties of cartilage in situ by combining atomic force microscopy stiffness mapping with immunofluorescence imaging of PCM and ECM regions in cryo-sectioned tissue samples. This method was used to test the hypotheses that PCM biomechanical properties correlate with the presence of type VI collagen and are uniform with depth from the articular surface. Control experiments verified that immunolabelling did not affect the properties of the ECM or PCM. PCM biomechanical properties correlated with the presence of type VI collagen, and matrix regions lacking type VI collagen immediately adjacent to the PCM exhibited higher elastic moduli than regions positive for type VI collagen. PCM elastic moduli were similar in all three zones. Our findings provide further support for type VI collagen in defining the chondrocyte PCM and contributing to its biological and biomechanical properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.