ArticleYeast (Chichester, England)2012
A new method to efficiently induce a site-specific double-strand break in the fission yeast Schizosaccharomyces pombe.
Article in Yeast (Chichester, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2026Article
- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ccq1 restrains Mre11-mediated degradation to distinguish short telomeres from double-strand breaks.Nucleic acids research · 2024Article
- Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Review
- Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox.Frontiers in molecular biosciences · 2020Review
- Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast.Molecular cell · 2019Article
- Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.PLoS genetics · 2019Article
- XPG-related nucleases are hierarchically recruited for double-stranded rDNA break resection.The Journal of biological chemistry · 2019Article
- Histone H2A insufficiency causes chromosomal segregation defects due to anaphase chromosome bridge formation at rDNA repeats in fission yeast.Scientific reports · 2019Article
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- Pericentromere-Specific Cohesin Complex Prevents Meiotic Pericentric DNA Double-Strand Breaks and Lethal Crossovers.Molecular cell · 2018Article
- A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.Molecular and cellular biology · 2018Article
- RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.EMBO reports · 2018Article
- Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair.Nucleic acids research · 2015Article
- Initiation of DNA damage responses through XPG-related nucleases.The EMBO journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Double-strand DNA breaks are a serious threat to cellular viability and yeast systems have proved invaluable in helping to understand how these potentially toxic lesions are sensed and repaired. An important method to study the processing of DNA breaks in the budding yeast Saccharomyces cerevisiae is to introduce a unique double-strand break into the genome by regulating the expression of the site-specific HO endonuclease with a galactose inducible promoter. Variations of the HO site-specific DSB assay have been adapted to many organisms, but the methodology has seen only limited use in the fission yeast Schizosaccharomyces pombe because of the lack of a promoter capable of inducing endonuclease expression on a relatively short time scale (~1 h). We have overcome this limitation by developing a new assay in which expression of the homing endonuclease I-PpoI is tightly regulated with a tetracycline-inducible promoter. We show that induction of the I-PpoI endonuclease produces rapid cutting of a defined cleavage site (> 80% after 1 h), efficient cell cycle arrest and significant accumulation of the checkpoint protein Crb2 at break-adjacent regions in a manner that is analogous to published findings with DSBs produced by an acute exposure to ionizing irradiation. This assay provides an important new tool for the fission yeast community and, because many aspects of mammalian chromatin organization have been well-conserved in Sz. pombe but not in S. cerevisiae, also offers an attractive system to decipher the role of chromatin structure in modulating the repair of double-stranded DNA breaks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.